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bss manager resume Resume keywords summary: IT Manager, Billing Manager, Project Manager, Program Manager, General Manager. Profile/Cover Letter: Objective. Experienced professional with a career dominated by the successful delivery of full lifecycle technology projects. 15 years of total experience, 14 years of exp in back valuation the Telecom IT Industry. 10 years of Management Experience including Project Management, Delivery, Operations Support. Have experience in Help Development, Implementations, Integration, Migration, Cutover Transition. Having good idea about planning of money back valuation projects, understanding of client requirement, scoping of How to motivation a paper project etc. Well versed in Onshore-Offshore model. Money Back Valuation. Good Understanding of Client-Relationship Management, SLAs.

Having hands on experience in the process and implementing those plans for smooth transitioning new solution with production ready data. Experience in Various Telecom Billing systems INs -- @billity, BSCS 5.2/6.0, ISIS, Singl.eView, Siemens IN 6.2/7.5, Comverse IN, Service Node Platform, understanding of Help in essay writing Mediation device (Wipro, Comptel, EMM), CRM, EBPP and other Surround Applications. Essay Money Back Valuation. Good knowledge of GSM CDMA Telecom Operations. March 2011 till date Tech Mahindra Limited Sr. Project Manager. June 2010 March 2011 Tecnotree Convergence Limited Sr. Project Manager. September 2007 March 2010 Patni Computer Systems Limited Manager Software ( Project Manager) February 2007 August 2007 Multi-Links Communications, Lagos Manager Billing.

November 2004 February 2007 Hutchison Essar Limited Manager IT. February 2001 September 2004 Madacom SA, Madagascar IT Billing Manager. July 1997 January 2001 Lifetree Convergence Limited Software Engineer. June 1996 June 1997 Span Computer Education Teaching Faculty. RAD Requirement Analysis and Design. C S Configuration Support. SIT System Integration and Testing. A Compare And Contrast On Dogs. UAT User Acceptance Testing. Project Management of all the ongoing projects. Development through delivery stream.

Compliance Management Release Management, Change control, Baseline Control. June 2010 March 2011. Senior Project Manager, Tecnotree Convergence Limited, India. Successfully delivered BSS Project, CRM project Loyalty Project for Malawi Telecommunications MTN-Uganda. Successfully initiated Convergence project for MTN-Group Uganda, Rwanda Swaziland. Money Back Valuation. Successfully initiated Hub Spoke concept for MTN- Group Zambia, Rwanda Swaziland at Uganda. Program managed PoC for Claro Latin America BSS project.

Project Management which is homework for me homework, worked on onsite-offshore model. Single point of contact for back valuation, the customer for all requirements of the project. Developing project plans and ensuring risks are mitigated effectively to ensure quality and timely delivery of the project. Study and Analysis Studying the legacy billing system at MTN and reading analyze the migration impact and addressing the Gaps. Interacting with other streams to discuss the status of the project, clarify any queries regarding the functionality etc. Interfacing with customers with respect to project delivery and ensuring customer requirements are adequately factored in the project on an on-going basis. Planning project work and determining critical path items and ensuring these are always on track. Data Comparison of migrations, system tests, dry runs, test runs of Rating Billing. Decide on Essay, important milestones for Data Migration, participate in negotiation for Do my homework for me assignments, SLAs. Decide on acceptance criteria for Essay money, various deliverables.

Ensure quality is of deliverables. In Essay. Estimating resource needs and coordinating with other functions. Essay Valuation. Responsible for development of for me team members by review, feedback and Essay valuation coaching. Project Wholesale Billing System Operations (Singl.eView) Client MTN South Africa. Role Production Support Manager. Duration September 2009 March 2010. English And Writing. Project PowerBill Phase 2 Data Migration. Client MTN South Africa. Back. Role Project Manager. Duration September 2007 August 2009. Project Unified Prepaid Support System (UPSS) Client Vodafone - India.

Role Data Migration Cutover Manager. Duration September 2008 January 2009. Feb 2007 Aug 2007. Manager Billing IT, Multi-Links Telecom Ltd, Lagos, Nigeria. Nov 2004 Feb 2007. Manager IT, Hutchison Essar Limited, Mumbai, India. Migration of BSCS 5.2 to BSCS 6.0 for 3 new circles of Hutch. Rolling of India One Roaming Product. Standardization of a compare essay Business processes across all Hutch circles. Purging of older data for Essay money, Mumbai circle. Changes undertaken during the implementation of Comptel Mediation.

Feb 2001 Sept 2004. In Writing Includes Your. Billing IT Manager. Madacom SA (Celtel Zain Airtel), Madagascar. July 1997 to Jan 2001. Software Engineer. Lifetree Convergence Ltd, Bangalore, India. Jan 2000 - Jan 2001.

Telecom Billing Accounting System (@billity) Project involved developing a Telecom Billing and Accounting System for the Client Spice Telecom Bangalore. I was responsible for developing Customer care module, Billing module, Mediation part, reports module TAP module. I was in-charge for documentation testing of the entire application and data migration from the money legacy billing system to Critically in writing, @billity. July 98 to June 99 Madacom Telecom, Madagascar. Essay Back. Dec 97 to May 98 Spice Telecom, Chandigarh, India. July 97 to Nov 97 Under Training @ Spice Telecom, Bangalore, India. Responsibilities include developments debugging as per the operational needs providing various reports. Generation of invoices and providing the output files for printing of invoices in the format required for printing.

Evaluate new tariffs or products and Things to write implement in the billing system. Development of operational reports for various departments. Developments of additional modules like IVR interface TAP for Essay, roaming. Provide training hygienic education to end-users. In Writing Includes. Ensure the high availability of the systems to end-users. Maintenance of the accounting software and support accounts department.

Jun 1996 to Jun 1997. Teaching Faculty. Span Computer Education, Bangalore India.

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resume pfleiderer ru WO 2005113829 A2. USE OF WHOLE BLOOD IN PCR REACTIONS. [01] BACKGROUND [02] The polymerase chain reaction (PCR) is Essay money valuation, a sensitive DNA amplification procedure that permits the selection and detection of specific nucleic acids from a complex mixture. In its most rudimentary form, PCR is employed using a sample that contains a target nucleic acid (DNA), a set of DNA primers that hybridize to the target, and a DNA polymerase that is capable of find to write, primer-based synthesis of complementary strands of the target. During the nucleic acid amplification process, the target:primer:polymerase mixture is money back valuation, subjected to successive rounds of heating at different temperatures to facilitate target DNA strand separation (performed at -90-99° C), prime?target DNA strand annealing (performed at -40-70° C), and DNA polymerase-mediated primer elongation (performed at.

50-72° C) to create new complementary target strands. Includes! Because the reaction may be subjected to -25-45 rounds of cycling to yield the desired DNA amplification product, PCR is usually conducted using thermal stable DNA polymerases. Money Back Valuation! that can withstand the very high temperatures associated with target strand separation without suffering inactivation due to heat-induced protein denaturation. Critically Thinking In Writing Includes Your! Since its introduction in the mid-1980's, PCR has become the de facto standard for detecting minute quantities of nucleic acids in samples, and obtaining specific genes from complex DNA genomes and samples. [03] A major problem with diagnostic and forensic techniques based on PCR is the false-negative reactions or low sensitivity caused by Essay money inhibitory substances that interfere with PCR (1, 2, 3). Of particular clinical importance is the PCR analysis of writing assistance letter, blood samples, which represents the largest fraction of human health related tests for diagnosis of genetic diseases, virus and microbial infections, blood typing, and safe blood banking. Various studies indicate that the inhibitory effect of blood on Essay money PCR is primarily associated - with direct inactivation of the thermostable DNA polymerase and/or capturing or degradation of the target DNA and primers. Do My Homework For Me Assignments! It has been reported that the protease activity in blood also contributes to the reduced efficiency of PCR (1-5, 7, 10, 12). [04] The blood resistance characteristics of the thermostable DNA polymerases vary with the source of the enzyme (6). Widely used thermostable polymerases like Thermus aquaticus DNA polymerase (Taq) and AmpliTaq Gold ® are completely inhibited in the presence of 0.004-0.2% whole human blood (vol/vol; 3, 4, 6). Essay! Various agents have been tested for reducing the inhibitory effect of Help writing, blood on Taq.

It was found that an money back valuation addition of betaine, bovine serum albumin, the single-stranded DNA binding protein of the T4 32 gene (gp 32), or a cocktail of protease inhibitors can partially relieve the Do my homework assignments make, blood inhibition and allow Taq to work in up to 2% blood (vol/vol), although this effect could be sample specific (3, 8, 9, 1 1). Essay Back Valuation! [05] Several major inhibitors of PCR in human blood have been characterized such as immunoglobulin G, hemoglobin, lactoferrin and excess of leukocyte DNA (4, 7, 10). The IgG, hemoglobin, and lactoferin have been purified from plasma, erythrocytes and leukocytes, respectively, using size-exclusion and anion-exchange chromatography (4, 7). Help Writing! The heme has been reported to Essay money, inactivate the Taq polymerase by binding to its catalytic domain (10), while the mechanism of to write and contrast essay on dogs, action of the other inhibitory components is more poorly understood. Essay! The inhibitory effect of IgG can be reduced when this plasma fraction is heated at 95° C before adding it to PCR, or with the addition of Do my assignments make, excess non-target DNA to the PCR mixture. However, heating of IgG together with target DNA at 95° C was found to block amplification. Inhibition by IgG may be due to an interaction with the single-stranded DNA fraction in the target DNA. The inhibitory effect could be removed also by treating the plasma with DNA-agarose beads prior to amplification (4). [06] Other complicating factors include EDTA and heparin, used as anti-coagulants, which can also inhibit DNA amplification. The addition of money, heparinase has been shown to counteract the heparin-mediated inhibition (13, 14). Therefore, various laboratory procedures of sample preparation have been developed to reduce the Critically thinking in writing includes, inhibitory effect of Essay money back valuation, blood. The DNA purification methods suitable for PCR can include additional steps like dialysis, treatment with DNA-agarose beads or Chelex 100 resin, multiple DNA washes, or a combination of dilution with buffer which causes lysis of red blood cells, centrifugation to recover the white blood cells, washing with NaOH and the addition of bovine serum albumin (2,3, 15-19). [07] These pre-treatment steps of the blood samples are generally time-consuming, labor-intensive, and can be sample specific. The guanidinium thiocianate method for.

DNA isolation is not suitable for Resume writing cover reliable detection of Mycobacterium tuberculosis in clinical samples. An alternative method of money back, DNA purification with protease K treatment followed by phenol-chloroform extraction has to be employed to relieve the inhibition. (20). Critically In Writing Includes! Separation with a QIAamp kit followed by money back valuation dialysis with a Millipore filter are required for to write a compare and contrast essay eliminating the heme inhibition of hepatitis B virus detection (21). In addition, some the above steps carry a risk of money, target DNA losses and are not suitable for Critically your automation. Moreover, even commercial kits specially formulated for DNA purification from blood samples such as QIAmp or GeneReleaser are not always satisfactory. The reason is due to valuation, an incomplete removal of Taq inhibitors, which can result in false- negative results. For example, 14% of the human blood samples tested for hepatitis B virus yielded false-negative results when using such blood kits (21). In Essay Kalä±plarä±! [08] The objective of achieving specificity of money valuation, amplification reactions for samples containing whole blood is further complicated by two types of unwanted DNA synthesis reactions that occur during PCR. Both types of side-reactions are frequently competitive with the find to write a paper, desired target and Essay money back, can lead to impure product or failed amplification. This is particularly problematic for PCR assays containing a low copy number of the nucleic acid template target, wherein the PCR conditions are modified to include a greater number of amplification cycles to achieve an adequate yield of the desired amplification product. [09] The first type of unwanted DNA synthesis is priming on less specific sequences in the template.

This is only an issue if the template is contaminated with single-stranded nucleic acid or if the template is in writing, single-stranded, which is the case if the DNA preparation has been subjected to Essay, melting conditions during its isolation. [010] The second type of unwanted DNA synthesis is writing assistance letter, primers acting as templates for themselves and/or each other, with at least the result of modifying their 3' ends by the addition of back valuation, additional nucleotides. These so-modified primers are able to Do my for me, anneal to the nucleic acid target; however, they do not serve as primers for complementary strand synthesis due to the presence of mismatched nucleotides at the site of elongation between the money valuation, 3' end of the primer and the desired target. This problem is often referred to as. primer dimer, although this name is not accurately descriptive. This problem can often be reduced or avoided by a paper careful primer design, and it is more of money back, a problem with multiplex PCR, since there is more opportunity for accidental homology among multiple pairs of Critically thinking your, primers. [Oil] A procedure known as hot start PCR avoids the occurrence of both types of unwanted DNA synthesis side-reactions. According to valuation, this method, the enzyme DNA polymerase, or a buffer component essential to its activity, such as the magnesium (II) cation and/or the dNTPs, is English reading and writing past, withheld from the other PCR assay mixture ingredients until the PCR reaction has been heated to at least the normal primer-annealing (or, preferably, the DNA extension) temperature (55-75° C, optimal 68° C). At this temperature the primers can presumably not form stable duplexes with themselves or at unwanted template sequences.

After the Essay money back valuation, selective temperature is achieved, the omitted component is added to reaction to writing letter, reconstitute a functional amplification mixture. [012] Typical hot start PCR procedures are not only Essay valuation, labor-intensive, they expose the PCR reactions to contamination with each other and with molecules that have been previously amplified in the thermal cycler machine. [013] The more standard ways of executing a hot start consist of formulating the PCR reaction in in writing two parts, such that the DNA polymerase is not able to act on the DNA until the Essay back valuation, two portions are combined at high temperature, usually 65-85° C. For instance, an initial solution containing all of the magnesium is introduced to the reaction tube encapsulated in How to to write a paper a wax bead or sealed under a layer of wax. The rest of the reaction, without Mg, is then added, along with an overlay of oil, if appropriate. While the reaction heats for the first cycle, the wax melts and Essay money valuation, floats to Things to write and contrast essay on dogs, the surface, allowing the magnesium to mix with the reaction volume. Essay Money! The DNA polymerase activity is therefore reconstituted at a temperature that does not allow non-specific or unwanted primer interactions. A great drawback to the wax method comes after the PCR cycling is Critically thinking includes your, complete, and the product must be withdrawn for money back valuation analysis. The wax then tends to plug the pipette tip, greatly adding to the time and effort of in essay, reaction analysis. [014] Recently, a method of hot start which is not hot at all, but which uses anti-Taq antibodies, has been described, patented and made commercially available (33-35). The antibodies largely neutralize the enzyme activity of the Taq polymerase, and can be added any time prior to the primers, or be conveniently present during storage of the stock enzyme.

The antibodies are thermolabile, thus permitting the back valuation, Taq polymerase to resume activity after the first heat step. The antibodies so far developed for this method must be used in 10-fold molar excess and are expensive. How To Find A Paper! Furthermore, the antibodies inhibit some long PCR assays that are conducted with the KlentaqLA polymerase mixture. [015] A chemically inactivated form of the money valuation, Taq polymerase has been introduced recently, termed AmpliTaq Gold ® . The nature of the inactivation is proprietary, but the inactivation is reversible by heating the polymerase at 95° C. This method may be even more convenient than the other methods, but it has at in essay writing, least one current disadvantage: the time for reactivation is about 10 minutes at Essay money valuation, 95° C. This procedure is homework for me make, incompatible with long PCR applications, as this treatment would excessively depurinate nucleic acid targets longer than a few kb. [016] Thus, the analysis of whole blood samples using PCR would be benefited by the discovery of new reagents and methods that overcome the aforementioned shortcomings of current PCR technologies. Essay Money Back Valuation! The invention disclosed herein addresses and solves many of these shortcomings. [018] In a first aspect, the present invention is a method of obtaining DNA amplification of a nucleic acid target from past, a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase. [019] In a second aspect, the present invention is method of obtaining DNA amplification of a nucleic acid target from Essay back valuation, a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with KT-1 (SEQ ID NO:2) or Z-TAQ™ [020] In a third aspect, the present invention is a method of reading and writing, obtaining DNA amplification of a nucleic acid target from a whole blood sample with a DNA amplification cocktail by avoiding mixing of the whole blood sample with the Essay money back valuation, DNA amplification cocktail in Resume assistance cover a reaction vessel before thermal cycling that includes the following steps: adding the DNA amplification cocktail to the reaction vessel, wherein the DNA amplification cocktail comprises at least one DNA polymerase; adding the Essay back, whole blood sample to the reaction vessel, wherein the whole blood sample is layered beneath the DNA amplification cocktail regardless of the order of addition of the DNA amplification cocktail and the whole blood sample to the reaction vessel; and performing a thermal cycling program to effect DNA amplification of the nucleic acid target. [021] In a fourth aspect, the in essay kalä±plarä±, present invention is a method of obtaining a hot start for DNA amplification of a nucleic acid target that includes the Essay back, preparation of the reaction cocktail comprising at least a first volume component and a second volume component. The second volume component is heavier than the first volume component.

The first volume component comprises a DNA polymerase cocktail lacking an essential constituent required for in writing your DNA amplification activity. The second volume component includes the essential constituent required for DNA amplification activity. The second volume component is underlayed below the first volume component without undue mixing before a DNA amplification reaction is initiated. [022] In a fifth aspect, the present invention is an money back isolated polypeptide comprising an amino acid sequence having at least 80% amino acid sequence identity with at Resume writing, least one member selected from the group consisting of KT-6 (SEQ ID NO:4), KT-7 (SEQ ID NO:6), KT-10 (SEQ ID NO:20), KT-12 (SEQ ID NO:24), FL-10 (SEQ ID NO:28), and FL-12 (SEQ ID NO:30), wherein the money back valuation, isolated polypeptide comprises a blood-resistant polymerase. [023] In a sixth aspect, the present invention is an How to isolated polypeptide comprising a nucleotide sequence having at least 80% amino acid sequence identity with at least one member selected from the group consisting of KT-7 (SEQ ID NO:6), KT-11 (SEQ ID NO:22), KT-12 (SEQ ID NO:24), and Essay, FL-12 (SEQ ID NO:30), wherein the How to a paper research, isolated polypeptide comprises a faster elongating polymerase. Essay Back Valuation! [024] In a seventh aspect, the Critically thinking includes your, present invention is an isolated polypeptide comprising at least one member selected from the Essay money back valuation, group consisting of writing, KT-6 (SEQ ID NO:4), KT-7 (SEQ ID NO:6), KT-10 (SEQ ID NO:20), KT-11 (SEQ ID NO:22), KT-12 (SEQ ID NO:24), FL-10 (SEQ ID NO:28), and FL-12 (SEQ ID NO:30). [025] In an eighth aspect, the money valuation, present invention is an isolated polypeptide comprising KT-1 (SEQ ID NO:2) having at Help kalä±plarä±, least two amino acid residue substitutions, wherein one of Essay money, the at least two amino acid residue substitutions comprises amino acid residue position 430 such that the isolated polypeptide encodes a blood-resistant polymerase, a faster elongating polymerase, or a blood-resistant, faster elongating polymerase. [026] In a ninth aspect, the present invention is an isolated polypeptide comprising Taq. DNA polymerase (SEQ ID NO:26) having at least three amino acid residue substitutions, wherein one of the at in essay writing, least three amino acid residue substitutions comprises amino acid residue position 708 such that the isolated polypeptide encodes a blood-resistant polymerase, a faster elongating polymerase, or a blood-resistant, faster elongating polymerase. [027] In a tenth aspect, the present invention is an isolated nucleic acid comprising a nucleotide sequence having at least 80% nucleotide sequence identity with at least one member selected from the group consisting of KT-1 (SEQ ID NO:l), KT-6 (SEQ ID NO:3), KT-7 (SEQ ID NO:5), KT-10 (SEQ ID NO: 19), KT-12 (SEQ ID NO:23), Taq DNA polymerase (SEQ ID NO:25), FL-10 (SEQ ID NO:27), and FL-12 (SEQ ID NO:29), wherein the isolated nucleic acid encodes a blood-resistant polymerase. [028] In a eleventh aspect, the present invention is an isolated nucleic acid comprising a nucleotide sequence having at least 80% nucleotide sequence identity with at least one member selected from the group consisting of KT-1 (SEQ ID NO:l), KT-7 (SEQ ID NO:5), KT-11 (SEQ ID NO:21), KT-12 (SEQ ID NO:23), Taq DNA polymerase (SEQ ID NO:25), and Essay, FL-12 (SEQ ID NO:29), wherein the isolated nucleic acid encodes a faster elongating polymerase.

[029] In a twelfth aspect, the present invention is an isolated nucleic acid comprising at thinking, least one member selected from the group consisting of KT-6 (SEQ ID NO:3), KT-7 (SEQ ID NO:5), KT-10 (SEQ ID NO:19), KT-1 1 (SEQ ID NO:21), KT-12 (SEQ ID NO:23), FL-10 (SEQ ID NO:27), and Essay back valuation, FL-12 (SEQ ID NO:29). [030] In a thirteenth aspect, the present invention is an isolated nucleic acid comprising KT-1 (SEQ ID NO:l) having at motivation a paper research, least two codon substitutions, wherein one of valuation, the at least two codon substitutions comprises codon position 430 such that the isolated nucleic acid encodes a blood-resistant polymerase, a faster elongating polymerase, or a blood- resistant, faster elongating polymerase. Help In Essay Writing! [031] In a fourteenth aspect, the present invention is an Essay valuation isolated nucleic acid comprising Taq DNA polymerase (SEQ ID NO:25) having at least three codon substitutions, wherein one of reading and writing, the at money, least three codon substitutions comprises codon position 708 such that the isolated nucleic acid encodes a blood-resistant polymerase, a faster elongating polymerase, or a blood-resistant, faster elongating polymerase. [032] In a fifteenth aspect, the present invention is a method of obtaining rapid DNA amplification of a nucleic acid target in a PCR assay mixture comprising a faster elongating DNA polymerase. [033] In a sixteenth aspect, the present invention is a kit for performing PCR assays on samples of whole blood, wherein the kit comprises a blood-resistant polymerase. Critically Thinking Includes Your! [034] In a seventeenth aspect, the Essay money valuation, present invention is a kit for performing PCR assays on samples of whole blood, wherein the kit comprises KT-1 (SEQ ID NO:2) or Z-TAQ™. [036] The term amplicon refers to How to find research, the nucleic acid that is the Essay, target of DNA amplification of a PCR assay. [037] The phrase amplification activity refers to the functional ability of a DNA polymerase to synthesize copies of a nucleic acid target under the PCR conditions disclosed herein to yield a quantity of amplified DNA product that is discernable by intercalative dye (e.g., ethidium bromide) staining methods that are well known in the art. Do My Assignments Make! [038] The phrase homogeneous PCR assay solution as used herein refers to a solution that is homogenous with respect to the absence of discrete phases. A homogeneous PCR assay solution is one that is typically prepared by back valuation mixing the contents of a reaction vessel using a vortexer or comparable mixing apparatus. Includes! In the back, context of heavy hot start PCR assays, the PCR assay solution is find to write a paper research, composed of two phases prior to initiating the thermal cycling program; that is, the PCR assay solution of a heavy hot start PCR assay is not premixed prior to money back, initiating a thermal cycling program and is not considered a homogenous PCR assay solution. [039] The phrase blood-resistant polymerase as used herein refers to a mutant form of either Klentaq-278 DNA polymerase or full-length Taq DNA polymerase wherein the mutant enzyme is cold sensitive and displays amplification activity in a homogeneous.

PCR assay solution containing whole blood in the range from about 3% (vol/vol) to about 25% (vol/vol). By cold sensitive, the mutant enzyme displays lower amplification activity than wild-type Taq DNA polymerase at reduced temperatures relative to thinking, the normal temperature at back, which DNA extension reactions are performed (-72 °C). Things To Write A Compare On Dogs! Such a mutant enzyme displays DNA amplification activity under hot start PCR conditions. A mutant form of Klentaq-278 DNA polymerase includes a polypeptide that does not encode the Essay money back, identical amino acid sequence of Klentaq-278 DNA polymerase (SEQ ID NO:2). Examples of motivation research, such mutant forms include a deletion of at least one amino acid, an insertion of additional amino acids, or a change of at least one amino acid relative to the amino acid sequence of the back valuation, Klentaq-278 DNA polymerase (SEQ ID NO:2). A mutant form of full-length Taq DNA polymerase includes a polypeptide that does not encode the identical amino acid sequence of the Things a compare and contrast essay on dogs, full-length Taq DNA polymerase (GenBank Accession No.

J04639; SEQ ID NO:25). Money Back Valuation! Examples of such mutant forms include a deletion of at least one amino acid, an reading past insertion of back valuation, additional amino acids, or a change of at least one amino acid relative to a compare, the amino acid sequence of the full-length Taq DNA polymerase (SEQ ID NO:25). [040] The phrase faster elongating polymerase as used herein refers to a derivative of Taq DNA polymerase that displays amplification activity in money back PCR assays conducted with extension times in Resume assistance with cover letter the range from about 12 seconds to about 50 seconds to complete up to 2 kb. [041] The phrase physiologically compatible buffer as used herein refers to any solution that is money back, compatible with the a compare and contrast essay on dogs, function of money, enzyme activities and and contrast, enables cells and biological macromolecules to retain their normal physiological and biochemical functions. Typically, a physiologically compatible buffer will include a buffering agent (e.g., TRIS, MES, PO 4 , HEPES, etc.), a chelating agent (e.g., EDTA, EGTA, or the like), a salt (e.g., NaCl, KCl, MgCl 2 , CaCl 2 , NaOAc, KOAc, Mg(OAc) 2 , etc.) and optionally a stabilizing agent (e.g., sucrose, glycerine, Tween20, etc.). [042] The polymerases referred to throughout this description have the following structures and properties: (1) Taq refers to Essay back valuation, the wild-type, full-length DNA Polymerase from Thermus ?qu?ticus (GenBank Accession No. J04639) and also used for chemically modified variants thereof, such as AmpliTaq Gold ® ; (2) Klentaq-235 refers to an N- terminal deletion of the first 235 amino acids of Taq. Klentaq-235 is homework assignments, also known in valuation commerce as DeltaTaq, ?Taq, Klentaq, and Klentaq5; (3) Klentaq-278 refers to an N- terminal deletion of the first 278 amino acids of Taq (Klentaq-278 is also referred to as Klentaql or KT-1 or wild-type Klentaql) and is described in claims 1-5 of U.S. Patent No. 5,436,149; (4) Klentaq? (abbreviated as KT-6) refers to Klentaq-278 with two amino-acid changes; (5) Klentaq7 (abbreviated as KT-7) refers to Klentaq-278 with three amino-acid changes; (6) Klentaq 10 (abbreviated as KT-10) refers to Klentaq-278 with three amino acid changes; Klentaql 1 (abbreviated as KT-11) refers to Klentaq-278 with four amino acid changes; Klentaq 12 (abbreviated as KT-12) refers to Klentaq-278 with four amino acid changes; FL-10 refers to full-length Taq polypeptide with three amino acid changes; and FL-12 refers to full-length Taq polypeptide with four amino acid changes. These codon changes of the relevant Taq polymerase mutants are summarized in abbreviated form in Table I.

Table I. Codon changes in Taq DNA polymerase mutants. Odd- and even-numbered SEQ ID NOs refer to nucleic acid and polypeptide sequences, respectively, as illustrated in the Sequence Listing. 2 Wild-type (WT) base of top (codon) strand on the left, mutant (MUT) base on the right, of numerical positions of English reading past papers, changes (nuc) which numbers are in reference to the full-length Taq DNA polymerase encoding nucleic acid and polypeptide (herein SEQ ID NOs: 25 26, respectively; disclosed in GenBank Ace. Money! No. Do My For Me Homework Assignments Make! J04639); parenthetical numbers refer to the corresponding Klentaq-278 sequence positions (herein SEQ ID NOs:l 2, respectively; disclosed in money valuation U.S. Resume Writing Assistance! Patent No. Essay Money Valuation! 5,436,149).

3 Phenotype that was conferred when this mutation was added to its parent; CS, cold sensitive; BR, blood- resistant; FAST, fast DNA extension. * In the cases of homework assignments make, KT-7, KT-12, and its respective FL-version, both BR and Essay money, FAST phenotypes are present, presuming a possible double effect of these changes. Testing each mutation individually will clarify the linkage between the phenotypes. [043] The suffix LA means Long and Accurate and refers to a mixture of thermostable DNA polymerases, after claims 6-16 of English reading papers, US Patent 5,436,149 and Barnes (1994). Back! Major component is usually Taq or Klentaql. A minor component is usually an archaebacterial DNA polymerase such as Pfu polymerase, Pwu polymerase, Vent polymerase, or Deep Vent polymerase. [044] KlentaqLA is a mixture of 47: 1 : :Klentaql :Deep Vent by volume of commercially available enzymes. This mixture also may be modified to 24:1 as noted in the text.

Since commercially distributed Klentaql is about 15-20 times more concentrated than commercially distributed Deep Vent, the true ratio, by units or protein, is approximately 15-20 times higher, i.e., 705:1 or 360:1 [045] TaqLA is a mixture of 47:l::Taq:Deep Vent, or 16:l ::Taq:Pfu, or an unspecified mixture of Taq:Pfu that is commercially known as TaqPlus. [046] Control sequences are DNA sequences that enable the expression of an operably-linked coding sequence in a particular host organism. Prokaryotic control sequences include promoters, operator sequences, and ribosome binding sites. Eukaryotic cells utilize promoters, polyadenylation signals, and enhancers. Thinking In Writing Includes Your! [047] The phrase a reaction vessel refers to Essay money back, any container that may used for performing a biological, biochemical, or chemical reaction. In Essay Kalä±plarä±! In the context of PCR assays, a reaction vessel is Essay back, any suitable container that can withstand the temperatures carried out during a typical DNA amplification reaction. Preferably, a reaction vessel that used for PCR assays includes a tube fitted with a closure, wherein both the tube and the closure are made of and writing, polymeric material such as polypropylene or similar material commonly employed in the art. [048] The phrase isolated nucleic acid molecule is back, purified from the setting in which it is found in nature and is separated from at least one contaminant nucleic acid molecule. [049] The phrase isolated polypeptide molecule is purified from the setting in which it is found in nature and is separated from at least one contaminant polypeptide molecule. [050] The phrase purified polypeptide refers to a polypeptide molecule that has been purified to greater than 80% homogeneity by SDS-PAGE under non- reducing or reducing conditions using Coomassie blue or silver stain. Isolated polypeptides include those expressed heterologously in genetically engineered cells or expressed in homework make vitro. Ordinarily, isolated polypeptides are prepared by at Essay valuation, least one purification step. [051] BRIEF DESCRIPTION OF THE DRAWINGS. [052] Figure 1 depicts results of PCR assays with different forms of Klentaq polymerase (40 mutants and wild-type form of Klentaq) conducted in the presence of inhibitory amounts of blood. Clones KT-6 and in essay writing, KT-7 were capable of amplifying a 1.65 kbp target DNA from added plasmid template in the presence of 10% whole human blood; [053] Figure 2A depicts results of PCR amplification of a 0.32 kbp endogenous target DNA directly from whole blood with two mutant forms of Klentaq (KT-6 and KT-7) in the presence of increasing amounts of whole human blood (lane 1 : 0%; lane 2: 5%; lane 3: 10%; lane 4: 15%); [054] Figure 2B depicts results of PCR assays directed toward the Essay money valuation, amplification of a 0.32 kbp endogenous human Dystrophin gene fragment in the presence of the Help writing kalä±plarä±, indicated percentages of whole blood (vol/vol) in homogeneous PCR assay solutions with two mutant forms of Essay money back valuation, Klentaq (KT-10 and English reading and writing, KT-12), as shown above the figure; [055] Figure 2C depicts results of PCR assays directed toward amplification of a 1.1 kbp endogenous CCR5 gene fragment in the presence of the indicated percentages of whole blood (vol/vol) in homogeneous PCR assay solutions with two mutant forms of Klentaq (KT-10 and KT-12), as shown above the figure; [056] Figure 3 A depicts results of PCR assays directed toward the Essay money back valuation, amplification of a 0.32 kbp fragment of the endogenous human Dystrophin gene, 1.1 kbp and 2.5 kbp fragments of the endogenous human CCR5 gene, or a 4.3 kbp endogenous human Tissue Plasminogen Activator (TPA) gene fragment in How to to write a paper the presence of the indicated amounts of whole blood (vol/vol) (as shown above the figure) in homogeneous PCR assay solutions using a blood-resistant mutant form of full- length Taq DNA polymerase (FL-10) in comparison to blood-inactive commercial Taq enzymes (JumpStart™ Taq (Sigma), AmpliTaq Gold ® (Applied Biosystems) and Ex Taq™ (Takara)) (lanes denoted by 0 are PCR assays conducted in Essay money valuation the absence of blood and lanes indicated by 0+ refer to PCR assays conducted in the presence of 10 ng of human DNA); [057] Figure 3B depicts results of DNA amplification of for me, a 1.1 kbp endogenous CCR5 human gene fragment (indicated by the arrow) in money reactions of homogeneous PCR assay solutions containing whole blood at the indicated percentages (vol/vol) (as shown below the reading, figure) using FL-12 and Z-TAQ™ (Takara) Taq DNA polymerases; [058] Figure 4A depicts results of PCR amplification of a 1.65 kbp target DNA as a function of elongation time for reactions containing Klentaql polymerase (lane 1), two mutant Klentaq polymerases (KT-6 (lane 2) and KT-7 (lane 3)), and money back, another commercially available Taq polymerase (lane 4).

The extension times are indicated below the panel; [059] Figure 4B depicts results of PCR amplification of a 1.65 kbp target DNA as a function of exogenous template concentration and elongation time for reactions containing a mutant Klentaq polymerase (KT-7) and a DNA polymerase that possesses the highest prior art elongation rates (Z-TAQ™). The added nucleic acid target amounts were as follows: 0.5 ng (lane 1); 0.25 ng (lane 2); 0.125 ng (lane 3); and 0.06 ng (lane 4). The extension times were as follows: 60 sec (upper panel); 15 sec (central panel); and 12 sec (lower panel); [060] Figure 4C depicts results of How to find motivation research, PCR amplification of a 1.65 kbp target DNA (denoted by money arrow) using either mutant Klentaq DNA polymerases KT-7 (lane 1), KT-11 (lane 2), or KT-12 (lane 3), the wild-type Klentaq DNA polymerase (lane 4), the mutant full-length Taq DNA polymerase FL-12 (lane 5), or Z-TAQ™ (Takara; lane 6) performed with PCR cycles having extension steps reduced to 30 sec; [061] Figure 5 A illustrates the Resume assistance cover, results of heavy hot start PCR assays (100 ?l reaction volumes) conducted with KT-1 (SEQ ID NO:2), KT-6 (SEQ ID NO:4) and KT-7 (SEQ ID NO:6) in the presence of whole blood and under different conditions of Essay money, pre-treatment of the in writing your, reaction samples prior to initiating the thermal cycling program. The asterisks indicate those reaction vessels wherein the Essay money, heavy and light volume component layers were premixed by vortexing, i.e., reactions that a contain homogeneous PCR assay solution and that were not subjected to a heavy hot start procedure as described herein. Lanes 1-13, 15 and 17 are PCR assays directed toward the amplification of a 1.1 kbp target from the human CCR5 gene whereas lanes 14, 16, and 18 are PCR assays directed toward the amplification of Help, a 2.5kbp target from the human CCR5 gene; [062] Figure 5B illustrates an example of PCR assay tubes from reaction mixtures 9-14 of Fig. Essay Back Valuation! 5 A that were not mixed prior to initiating the thermal cycling reaction (heavy hot start reactions; reaction nos.

9,10, 13, and 14) or mixed by vortexing briefly prior to initiating the thermal cycling reaction (non- heavy hot start reactions; reaction nos. 11 and 12); [063] Figure 6 depicts results of heavy hot start PCR amplification of a 0.5 kbp target from the How to a paper, human CCR5 gene of cells present in whole blood. The reactions were conducted in the presence of whole blood in Essay the lower layer at the percentages indicated below each lane (vol/vol; adjusted for Critically your total volume of both layers), in the absence of whole blood (indicated by 0), or in absence of blood and in Essay money back the presence of 10 ng of in essay writing kalä±plarä±, human DNA (indicated by 0+); [064] Figure 7 depicts results of Essay money back valuation, PCR assays directed toward the and writing past, amplification of a 2.5kbp target from the human CCR5 gene derived from 2 ng of genomic DNA (designated DNA) or from 3% whole blood (vol/vol) (designated Blood) using either KT-1 (SEQ ID NO:2), KT-6 (SEQ ID NO:4), or KT-7 (SEQ ID NO:7) in Essay back valuation the absence of Deep Vent polymerase (lanes 1-3 and 7-9) or in the presence of to write, Deep Vent polymerase (lanes 4-6 and money valuation, 10-12), wherein the ratio of the KT enzyme to Deep Vent polymerase is about 360 to to write, 1. [065] DETAILED DESCRIPTION [066] The present invention makes use of the discovery that Taq polymerases bearing certain N-terminal deletions are unusually resistant to whole blood, making them ideally suited for use in Essay valuation analytical PCR assays of nucleic acid targets from human blood. Furthermore, mutant(s) of full-length Taq DNA polymerase with even higher resistance to blood inhibitors have been developed that remain fully functional in the presence of at least about 20-25% blood or the equivalent of Resume writing assistance, blood fractions. This level of blood tolerance exceeds that of the existing thermostable DNA polymerases (and even exceeds the amount of blood that can be practically or conveniently handled in the PCR analysis due to physical clumping). Moreover, mutants that display a high resistance to blood inhibitors have been identified that possess faster elongation rates. The use of these novel enzymes is expected to simplify and accelerate the performance of clinical and forensic tests as well as render such tests more sensitive and Essay valuation, economical. Finally, the present invention provides methods for enhancing DNA amplification specificity using these polymerases with samples from whole blood. In Writing! These Taq polymerase mutants and back, methods for their use are described below. [067] Identification of Klentaq mutants that are highly resistant to blood inhibition. [068] Klentaql polymerase (SEQ ID NO: 1 (nucleic acid) and SEQ ID NO:2.

(polypeptide)) is an improved and more robust version of the Taq polymerase that bears an N-terminal deletion of Things to write a compare, 278 amino acids from the full-length (832 amino acids) enzyme. Klentaql displays higher fidelity and back, greater thermostability than Taq. To Write A Compare And Contrast On Dogs! Klentaql is also inhibited to a lesser extent than Taq when the polymerase is used in Essay back valuation PCR assays carried out in the presence of blood products. For example, the purified Klentaql enzyme easily amplifies a nucleic acid target in the presence of about 5% whole blood in thinking reaction mixture (vol/vol). This was a highly unexpected result, as the full-length Taq enzyme is completely inhibited in Essay valuation a blood concentration range of about 0.004% to about 0.2% whole blood in the reaction mixture (vol/vol). No correlation between the N-terminal deletion of Taq, which generates Klentaql, and the blood resistance feature of the enzyme has been reported. [069] Several mutant Klentaq clones were analyzed by PCR assays for their ability to tolerate whole blood. About 40 mutagenized, yet PCR-functional Klentaq clones were constructed and Do my for me homework, tested in PCR assay mixtures containing about Essay money valuation, 10% whole human blood (vol/vol).

These 40 clones are cold sensitive or are mutants of clones whose enzyme product exhibited the cold sensitive phenotype. The cold sensitivity of the additional mutant clones has not yet been determined. Remarkably, two mutants of this small collection, KT-6 (SEQ ID NO: 3 (nucleic acid); SEQ ID NO: 4 (polypeptide)) and KT-7 (SEQ ID: 5 (nucleic acid); SEQ ID NO:6 (polypeptide)), clearly outperformed the rest of the Do my for me assignments, clones and the wild-type Klentaql protein under these conditions (Fig. 1). [070] These results were confirmed by performing PCR assays in Essay money the presence of thinking in writing includes, increasing amounts of whole blood. As shown in Figure 2A, clones KT-6 and Essay, KT- 7 remained functionally active in the presence of whole blood, being able to amplify an endogenous gene target directly from blood cells present in reactions containing about 15% whole human blood (vol/vol) without any DNA purification step.

The presence of assistance with, as little as 1% whole blood (vol/vol) in PCR assays was inhibitory for Taq (Roche) (see Example 4). Two additional mutant forms of Klentaq-278, clones KT-10 (SEQ ID NO: 19 (nucleic acid) and SEQ ID NO:20 (polypeptide)) and Essay money back, KT-12 (SEQ ID NO:23 (nucleic acid) and SEQ ID NO:24 (polypeptide)) also displayed the ability to amplify endogenous gene targets from whole blood samples (Figs. 2B and 2C). [071] The foregoing results reveal that whole blood may be used directly in Help kalä±plarä± screening assays to identify mutants of Klentaq-278 that are even more resistant to Essay money back valuation, blood. The present invention is drawn in a paper part to mutant forms of the Klentaq-278 DNA polymerase that display activity in PCR assays containing from about 5% whole blood to about 25% whole blood in the reaction mixture (vol/vol). More preferably, the invention is drawn to Essay valuation, mutant forms of the Klentaq DNA polymerase that display amplification activity in How to find to write a paper PCR assays containing from about 5% whole blood to about 20% whole blood in the reaction mixture (vol/vol), including 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, and 19%) whole blood in the reaction mixture (vol/vol). [072] Derivation of full-length Taq mutants that are highly resistant to back valuation, blood inhibition. [073] Because the mutant forms of Critically thinking your, Klentaq-278 were more robust polymerases in whole blood PCR assays than Klentaq-278, we considered it likely that the additional amino acid changes within the structure of this truncated Taq polypeptide might confer similar blood-resistant activities when incorporated into the full-length Taq enzyme. To test this hypothesis, the region of the KT-10 gene (SEQ ID NO: 19) that contains the relevant codon substitutions was re-introduced into the background of a wild-type full-length Taq (SEQ ID NO: 25 (nucleic acid) and SEQ ID NO:26 (polypeptide)) using standard recombinant DNA methods to yield the resultant mutant Taq gene, termed FL-10 (SEQ ID NO:27 (nucleic acid) and SEQ ID NO:28 (polypeptide)).

The resultant polypeptide was expressed and money back, tested in conjunction with other commercially available Taq polymerases in homogenous PCR assay solutions containing varying amounts of whole blood (0%, 10%, or 20% (vol/vol)). As is shown in in writing your Figure 3 A, FL-10 displays remarkably robust, blood-resistant, DNA amplification activity in Essay money back comparison with JumpStart™ Taq, AmpliTaq Gold ® , or Ex Taq™. [074] Similar to that found for FL- 10, another mutant form of Things a compare essay, full-length Taq DNA polymerase was identified that displayed high blood-resistant DNA amplification activity. This mutant was derived by cloning the region of the KT-12 (SEQ ID NO:23) that contains the relevant codon substitutions that impart blood-resistant DNA amplification activity to the KT-12 polymerase (SEQ ID NO:24) into valuation, the background of the wild-type full-length Taq (SEQ ID NO:25) using standard recombinant DNA methods to yield the How to find to write research, resultant mutant Taq polymerase, termed FL-12 (SEQ ID NO:29 (nucleic acid) and Essay money back valuation, SEQ ID NO:30 (polypeptide)). This full-length Taq polymerase mutant displayed blood-resistant DNA amplification activity that mirrors the activity observed for the KT-12 polymerase mutant (SEQ ID NO:24) (Fig. 3B). These findings provide evidence that the region of Do my homework for me homework make, any Klentaq-278 mutant that encodes blood-resistant DNA amplification activity will impart similar properties to the full-length Taq DNA polymerase when re-introduced into the context of the wild-type gene background. Money Valuation! [075] Although both FL- 10 and FL- 12 Taq polymerases displayed high blood- resistant DNA amplification activity, only the FL-12 Taq polymerase displays both faster-elongating activity and high blood-resistant activity. Writing Cover! Because these two properties are discrete attributes, we tested whether faster-elongating activity correlated with high blood-resistant activity. As described herein, Z-TAQ™ (Takara) is a proprietary form of the money, full-length Taq DNA polymerase that displays 5-fold faster elongation rates relative to Taq DNA polymerase. The nature of the alteration of Z-TAQ™ that is Help writing kalä±plarä±, responsible for its enhanced elongation activity is unknown in money back the art, owing to the fact that the kalä±plarä±, manufacturer of the enzyme regards Z-TAQ™ as a proprietary product. For this experiment, FL-12 Taq and Z-TAQ™ were evaluated for their respective blood-resistant DNA amplification activities.

As shown in Figure 3B, both FL-12 and Z-TAQ™ displayed blood-resistant DNA amplification activity in homogeneous PCR assay solutions, albeit the Essay back, FL-12 enzyme was more robust than Z-TAQ™ in reactions containing significant amounts of whole blood (20% (vol/vol)). [076] One functional characteristic that distinguishes the Help kalä±plarä±, aforementioned FL mutants ( . e. , FL- 10 and FL- 12) from money, Z-TAQ™ is assistance letter, that the FL mutants display a cold sensitive phenotype whereas Z-TAQ™ does not. One additional functional attribute that distinguishes the Essay valuation, aforementioned FL mutants from Z-TAQ™ is that the How to motivation a paper, FL mutants are capable of carrying out DNA amplifications under hot start conditions whereas the Z-TAQ™ polymerase lacks this capability. Money Valuation! Thus, whatever chemical or genetic attribute that endows Z-TAQ™ with its unusually high activity in whole blood PCR assays, it is not the identical modifications that render the in essay, FL mutants blood-resistant. For the purposes of this disclosure, blood- resistant DNA polymerases are defined to have three attributes: (1) display a cold sensitive phenotype in PCR assays relative to the wild-type Taq DNA polymerase; (2) display DNA amplification activity under hot start PCR conditions; and (3) display DNA amplification activity in Essay PCR assays containing whole blood in thinking the range from about 3% to Essay money back valuation, about 25% (vol/vol). [077] The foregoing results reveal that whole blood may be used directly in in essay writing kalä±plarä± screening assays to identify mutants of Klentaq-278 that are even more resistant to money back valuation, blood and that the methods are readily extendable to identifying mutants of full- length Taq that display blood-resistant DNA amplification activity. The present invention is Resume assistance cover letter, drawn in part to mutant forms of the full-length Taq DNA polymerase that display activity in back PCR assays containing from about 5% whole blood to about 25%o whole blood in the reaction mixture (vol vol).

More preferably, the invention is drawn to mutant forms of the full-length Taq DNA polymerase that display amplification activity in PCR assays containing from about 5% whole blood to about 20% whole blood in the reaction mixture (vol/vol), including 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, and 19% whole blood in the reaction mixture (vol/vol). [078] The presently preferred procedure for in essay writing identifying blood-resistant Taq DNA polymerases is to perform two screening operations on a collection of mutants: (1) identifying those mutants that display a cold sensitive phenotype in modified PCR assays; followed by (2) characterizing the subset of cold sensitive Taq DNA polymerase mutants for DNA amplification activity in whole blood PCR assays. Even more preferably, one may initially identify blood-resistant polymerases using an adaptation of Essay money, a selection procedure termed compartmentalized self-replication (25, 26) for obtaining DNA polymerase mutants with a predetermined activity. For Me Assignments Make! As illustrated in the Prophetic Example, one would initially select the Taq DNA polymerase mutant for its blood-resistant activity, followed secondarily by Essay a screening procedure to characterize its cold sensitive phenotype (e.g., DNA amplification activity under hot start PCR conditions). All mutants that are blood- resistant and homework for me make, display a cold sensitive phenotype would comprise members of the Essay back valuation, group of blood-resistant polymerases as defined herein. [079] Identification of Do my homework homework make, Klentaq and Taq mutants with faster DNA elongation rates [080] Rapid thermostable DNA polymerase mutants have been discovered that display a faster DNA elongation rate than found for the wild-type Klentaq-278 polymerase. By lowering the DNA extension times during PCR, certain PCR conditions have been determined where the elongation step in the cycle becomes limiting for successful amplification by the wild-type Klentaq-278 enzyme. In the case of using the back, Klentaq-278 gene as a target ( 1.65 kb long), the minimum extension time required was about 1 minute. How To To Write Research! For example, the Klentaq-278 polymerase did not possess amplification activity in back PCR assays performed under conditions that employ extension times of 50 seconds. English And Writing Past! Similar results were obtained with Taq enzyme. [081] About 40 functional mutant Klentaq clones were evaluated as a function of valuation, elongation rate. A 30 sec extension time was initially employed in the PCR assays, which reflect conditions that were found ineffective for the wild-type Klentaq and Resume assistance cover, AmpliTaq Gold ® . Essay Back! Interestingly, the mutants KT-6 (SEQ ID NO:4) and KT-7 (SEQ ID NO:6) were able to efficiently amplify the target with this shorter extension time (Fig.

4). Homework Homework Assignments Make! This feature of the two mutant enzymes was confirmed in further tests, wherein one of valuation, them (clone KT-7 (SEQ ID NO:6)) yielded amplification products with a 20 sec extension time (Fig. Help Writing! 4A). This enzyme feature was characterized further and yielded good amplification products even with 15 and money valuation, 12 sec extension times (Fig. How To Motivation To Write A Paper Research! 4B). Remarkably, the selected mutant completely outperformed the Essay, Z-TAQ™ (Takara) at How to find motivation to write research, these low extension times (Fig. 4B, central and lower panels). Similar results were obtained with two additional mutant forms of Klentaq-278, clone KT-11 (SEQ ID NO:21 (nucleic acid) and SEQ ID NO:22 (polypeptide)) and clone KT-12 (SEQ ID NO:23 (nucleic acid) and SEQ ID NO:24 (polypeptide)) (Fig.

4C). This is noteworthy because Z-TAQ™, a proprietary Taq enzyme, is one of the fastest DNA elongating PCR enzymes that is commercially available. [082] Because some of the mutant forms of Klentaq-278 were faster-elongating polymerases than observed for Klentaq-278, we considered it likely that the additional amino acid changes within the structure of this truncated polypeptide might confer similar faster elongating activities when incorporated into the full- length Taq enzyme. To test this hypothesis, the money back, region of KT-12 gene (SEQ ID NO:23) that contains the Help, relevant codon substitutions was re-introduced into the background of a wild-type full-length Taq (SEQ ID NO:25 (nucleic acid) and SEQ ID NO:26 (polypeptide)) using standard recombinant DNA methods to yield the resultant mutant Taq gene, termed FL-12 (SEQ ID NO:29 (nucleic acid) and SEQ ID NO:30 (polypeptide)). The resultant polypeptide was expressed and tested in conjunction with other commercially available Taq polymerases in homogenous PCR assay solutions using PCR conditions wherein the extension time was reduced to 30 sec. As is shown in back valuation Figure 4C, FL-12 displays remarkably robust, faster-elongating, DNA amplification activity in comparison to Z-TAQ™. [083] These results demonstrate that the elongation speed of the Klentaq DNA polymerase and the full-length Taq DNA polymerase can be improved by mutagenesis. The present invention is drawn in part to mutant forms of the Klentaq and full-length Taq DNA polymerases that display increased elongation rate in PCR assays under conditions where the a compare on dogs, respective enzymes fail to display successful amplification activity. Preferably, the invention is drawn to mutant forms of the Klentaq-278 and full-length Taq DNA polymerases that display amplification activity in PCR assays under conditions where the elongation step is time-limiting for Essay back the reaction with the a compare essay, wild-type Klentaq-278 polymerase.

Even more preferably, the Essay money, invention is Help in essay kalä±plarä±, drawn to mutant forms of money, Klentaq-278 and full- length Taq DNA polymerases that display amplification activity under PCR conditions disclosed herein and having extension times in the range from about 12 sec to about 50 sec, including 15 sec, 18 sec, 20 sec, 22 sec, 24 sec, 25 sec, 26 sec, 28 sec, 30 sec, 32 sec, 34 sec, 36 sec, 38 sec, 40 sec, 42, sec, 44 sec, 45 sec, 46 sec, and 48 sec. [084] Heavy hot start PCR procedures and applications to whole blood PCR [085] The new protocol described here uses no wax or antibodies, and requires no manipulations once the thermal cycling program has commenced. This protocol uses two aqueous layers at the time of setup of the PCR assay. Things To Write A Compare And Contrast Essay! The lower layer, which represents about 1/10 to about 1/4 of the final volume, includes the dNTPs and Essay valuation, magnesium(II) that is required for the reaction. The upper layer contains the English papers, polymerase enzyme, the primers, and the nucleic acid target. Both layers contain equivalent concentrations of other buffer components at the concentrations required for amplification. Essay Money Valuation! The lower layer also contains a constituent to homework, make it heavy, such as about 10-20% (wt/vol) sucrose, sorbitol or DMSO (or a suitable combination of similar reagents compatible with PCR up to about 10-20% (wt/vol)). [086] Optionally, other components that impart greater density to the lower layer may substitute for or supplement the items described above. Essay Money Valuation! For instance, Baskaran and How to find to write research, co-workers have demonstrated that 1.4 M betaine, 5% DMSO is money back valuation, good for PCR assays involving nucleic acid targets possessing high GC content (36).

These results suggest that inclusion of 2.8 M betaine, 10% DMSO is feasible as the heavy start component of the lower layer containing the MgCl 2 and Do my homework make, the dNTPs. Optionally and routinely, color in the form of money valuation, 0.05% cresol red is on dogs, also included in Essay the lower, heavy layer. [087] In reactions that include whole blood, the addition of components that impart greater density to includes, the lower layer and a color agent are not required. These features are superfluous because whole blood imparts a density to the lower layer that approximates that of the aforementioned heavy layer components and because the hemoglobin of blood provides color. In reactions containing whole blood, the template is included in the heavy layer, and all other components of the reaction are in the upper layer. The range of Essay money back valuation, volumes appropriate to the use of whole blood in the heavy layer comprises 1% to 25%. [088] Some adverse components of blood attack various components of the PCR reaction, such as the enzyme or the primers, yet the adverse components may be heat labile. Thus, the addition of the blood carefully as an Help in essay writing unmixed underlay allows it to valuation, be added without significant contact with the putatively sensitive PCR reaction components. Writing Cover Letter! Upon heating to normal PCR thermal cycling temperatures of 90-95° C, many of the blood components appeared denatured and aggregated in place, were visible as brown after the cycling, and either did not mix with the PCR components before being inactivated by the heat, or never did mix appreciably with the PCR reaction components.

Nevertheless, the genomic DNA template, and presumably other target templates such as viral and other microbial genomes, become timely available to Essay money valuation, the amplification reaction by convective mixing. [089] This principal of segregating heat labile inhibitors during reaction setup may have application to other situations of complex or environmental samples that do not involve blood. [090] The order of addition of the Things to write a compare essay, DNA polymerase cocktail and the whole blood sample to the PCR reaction vessel is not the critical aspect to the heavy hot start PCR procedure. Rather, the important aspect to the set-up of the heavy hot start PCR reaction is the careful addition of the DNA polymerase cocktail and the lower, heavier solution (e.g., a whole blood sample) to the PCR reaction vessel so as to avoid as little mixing of the individual layers of solutions as possible before thermal cycling begins. Essay Back! Thus, the lower, heavier solution can be initially added to the PCR reaction vessel, followed by the careful addition of the DNA polymerase cocktail as an overlayer. Things Essay! More preferably, however, the DNA polymerase cocktail is initially added to the PCR reaction vessel, followed by Essay money valuation the careful addition of the lower, heavy solution to writing cover letter, the PCR reaction vessel as an underlayer. [091] In the preferred embodiment, mixing of the layers occurs by Essay money back valuation diffusion and/or convection after the thermal cycler has warmed and cooled the reaction to find motivation to write, begin the PCR process. Essay Money Valuation! Layered reaction tubes containing whole blood that are experimentally premixed by vortex treatment are variably unable to Help writing, support PCR amplification activity, depending on the resistance of the reaction components, and the most sensitive component was discovered to be the DNA polymerase enzyme (Fig.

5 A). Figure 5B illustrates an back example of PCR assay tubes that contain discrete layers prior to reaction and the mixing of the layers during reaction. Reading And Writing Past Papers! [092] It is well understood to one of ordinary skill in the art that the valuation, combinations of components in the separate layers may be formulated in a variety of permutations. The only in essay writing kalä±plarä±, criteria that must be met in the present invention is Essay back, that the polymerase is separated from at reading papers, least one component essential to the amplification reaction (e.g., the primers, and/or the money, template, and/or Mg 2+ ), that the lower layer contains a component that imparts greater density to English, the solution, and that the mixing of the two layers results in reconstitution of the PCR assay conditions to permit amplification activity. [093] Because the inclusion of heavy reagents, such as sucrose, sorbitol or DMSO will decrease slightly the Essay valuation, melting temperature of the nucleic acid target, the denaturation step of the PCR cycle may have to Do my homework for me assignments, be reduced by money back valuation about 1-2 °C to compensate for this effect. [094] Mutant forms of Taq DNA polymerase include full-length Taq DNA polymerases that contain at least one amino acid change relative to the wild-type polypeptide (SEQ ID NO:26) encoded by the nucleic acid (SEQ ID NO:25) that are illustrated in the Sequence Listing.

Examples of such mutant forms of Taq DNA polymerase include FL-10 (SEQ ID NO:28) and FL-12 (SEQ ID NO:30). And Writing Papers! Additional mutant forms of back valuation, Taq DNA polymerase used in writing the invention include truncation mutants, such as Klentaq-278 that comprises the amino acid sequence (SEQ ID NO:2) encoded by the nucleic acid (SEQ ID NO:l) whose sequences are illustrated in the Sequence Listing, or other codons that encode those amino acids, or those amino acids with a few extra codons on the amino terminus thereof The invention also uses a mutant or variant gene encoding full-length Taq or Klentaq- 278, any of money back valuation, whose bases may be changed from the corresponding base shown in Help kalä±plarä± Tables 1-6 and 8-19 while still encoding a protein that maintains the activities and physiological functions of full-length Taq or of Klentaq-278, or a slightly longer or shorter version of Klentaq-278 at the N-terminus. Further included are nucleic acids whose sequences are complementary to those just described, including complementary nucleic acid fragments. Additionally, nucleic acids or nucleic acid fragments, or complements thereto, whose structures include chemical modifications, are also included. Essay Money Back Valuation! Such modifications include, by way of nonlimiting example, modified bases, and nucleic acids whose sugar phosphate backbones are modified or derivatized.

In the mutant or variant nucleic acids, and their complements, up to 20% or more of the bases may be so changed. [095] The invention also includes the Do my homework for me assignments, use of polypeptides and nucleotides having 80-100%) sequence identity to SEQ ID NOS: 1-6 and 19-30, including 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, and 99% sequence identity to SEQ ID NOS: 1-6 and 19-24, as well as nucleotides encoding any of these polypeptides, and complements of money back valuation, any of these nucleotides. In the case of Klentaql (SEQ ID NO:l), the invention includes mutant forms that contain at least one codon change in to write a paper research the open reading frame of Klentaql (SEQ ID NO:2). In the money back, case of Taq DNA polymerase (SEQ ID NO:25), the invention includes mutant forms that contain at least one codon change in the open reading frame of Taq DNA polymerase (SEQ ID NO:26). English Reading Papers! [096] Percentage Sequence Identity [097] Percent (%) nucleic acid sequence identity with respect to Klentaq-278- encoding nucleic acid sequences identified herein is back, defined as the percentage of nucleotides in a candidate sequence that are identical with the homework for me assignments, nucleotides in the Klentaq-278 sequence of interest, after aligning the sequences and Essay money back valuation, introducing gaps, if necessary, to achieve the maximum percent sequence identity. Resume Assistance With Letter! Alignment for purposes of determining % nucleic acid sequence identity can be achieved in various ways that are within the skill in Essay money the art, for instance, using publicly available computer software such as BLAST, BLAST-2, ALIGN or Megalign (DNASTAR) software. Those skilled in the art can determine appropriate parameters for measuring alignment, including any algorithms needed to achieve maximal alignment over for me assignments, the full-length of the sequences being compared.

The same methods and money back, principles apply to ascertain percent (%) nucleic sequence identity with respect to Taq DNA polymerase-encoding nucleic acid sequences in a candidate nucleic acid sequence when the two sequences are aligned. [098] When nucleotide sequences are aligned, the percent (%) nucleic acid sequence identity of a given nucleic acid sequence C to, with, or against a given nucleic acid sequence D (which can alternatively be phrased as a given nucleic acid sequence C that has or comprises a certain % nucleic acid sequence identity to, with, or against a given nucleic acid sequence D) can be calculated as follows: [099] % nucleic acid sequence identity = W/Z 100 [0100] where [0101] W is the number of nucleotides scored as identical matches by the sequence alignment program's or algorithm's alignment of C and D [0102] and. [0103] Z is the total number of nucleotides in D. [0104] When the English reading past papers, length of nucleic acid sequence C is not equal to the length of nucleic acid sequence D, the % nucleic acid sequence identity of C to D will not equal the % nucleic acid sequence identity of D to C. [0105] Percent (%) amino acid sequence identity is back valuation, defined as the percentage of amino acid residues that are identical with amino acid residues in the disclosed Klentaq-278 DNA polymerase polypeptide sequences in a candidate sequence when the Resume writing with, two sequences are aligned. Essay Back Valuation! To determine % amino acid identity, sequences are aligned and if necessary, gaps are introduced to achieve the maximum % sequence identity; conservative substitutions are not considered as part of the How to find motivation, sequence identity. Amino acid sequence alignment procedures to determine percent identity are well known to those of back valuation, skill in the art. Often publicly available computer software such as BLAST, BLAST2, ALIGN2 or. Megalign (DNASTAR) software is used to align peptide sequences. Those skilled in Things a compare and contrast essay the art can determine appropriate parameters for measuring alignment, including any algorithms needed to achieve maximal alignment over back, the full- length of the sequences being compared.

The same methods and thinking in writing includes, principles apply to ascertain percent (%) amino acid sequence identity with respect to Taq DNA polymerase-encoding polypeptide sequences in candidate sequences when the two sequences are aligned. [0106] When amino acid sequences are aligned, the % amino acid sequence identity of Essay money back valuation, a given amino acid sequence A to, with, or against a given amino acid sequence B (which can alternatively be phrased as a given amino acid sequence A that has or comprises a certain % amino acid sequence identity to, with, or against to write and contrast essay, a given amino acid sequence B) can be calculated as: [0107] % amino acid sequence identity = X/Y ' 100. [0108] where [0109] X is the Essay back, number of homework, amino acid residues scored as identical matches by the sequence alignment program's or algorithm's alignment of A and B. [0111] Y is the total number of amino acid residues in money valuation B. [0112] If the How to motivation to write research, length of Essay back, amino acid sequence A is not equal to the length of past, amino acid sequence B, the % amino acid sequence identity of money, A to B will not equal the. % amino acid sequence identity of reading and writing past, B to Essay money, A. [0113] A nucleic acid molecule used in Critically in writing the invention, e.g., a nucleic acid molecule having the nucleotide sequence of SEQ ID NOS:l, 3, 5, 19, 21, 23, 25, 27, or 29 or a complement of this aforementioned nucleotide sequence, can be isolated using standard molecular biology techniques and the provided sequence information. Using all or a portion of the nucleic acid sequence of SEQ ID NOS:l, 3, 5, 19, 21,

23, 25, 27, or 29 as a hybridization probe, Klent?q-278 or T?q gene molecules can be isolated using standard hybridization and cloning techniques (29, 30). [0114] PCR amplification techniques can be used to amplify Klent?q-278- or T?q- encoding DNA using Thermus aquaticus genomic DNA as a template and appropriate oligonucleotide primers. Furthermore, oligonucleotides corresponding to back, Klent?q-278 or T?q gene sequences can be prepared by Help writing kalä±plarä± standard synthetic techniques, e.g., an money back automated DNA synthesizer. [0115] Klentaq-278 is the subject of U.S. Patent No. 5,436,149 (31), which is incorporated herein by reference. Critically Thinking Your! [0116] Klentaq-235 is the subject of U.S. Patent No. 5,616,494 (32), which is incorporated herein by reference. [0117] Medical Applications. [0118] The applications of the present invention include diagnostic evaluations of whole blood samples for the presence and back, status of genetic disorders (e.g., cancer, blood disorders, diabetes, etc.) and diseases caused by blood borne microbial agents (e.g., viruses, bacteria, fungi, etc.); tissue-typing using polymorphisms, and forensic research. One of reading and writing, ordinary skill would recognize the utilities of blood- resistant polymerases and valuation, high elongating polymerases of the present invention toward advancing the application of PCR to whole blood samples directed to these objectives. [0119] Kits. [0120] The present invention also contemplates kits that may be employed in the clinical setting or in the field for permitting a simplified set of Critically thinking your, reagents for rapid PCR analysis of whole blood samples using the blood-resistant polymerases and high elongating polymerases of the Essay valuation, present invention.

Kits would typically include suitable oligonucleotide primers, PCR reaction buffer components, control solutions, and a suitable DNA polymerase, as well as instructions for the kit's use. Preferred DNA polymerase include the disclosed blood-resistant polymerases as defined herein (e.g., KT mutants that are blood-resistant and display a cold sensitive phenotype) as well as the Z-TAQ™ enzyme and the KT-1 enzyme (each of which displayed moderate blood resistance, but not cold sensitive). [0121] EXAMPLES [0122] Example 1. How To Motivation Research! Screening of mutagenized Klentaq clones for Essay money back valuation blood-resistant mutant enzyme activity. [0123] In order to functionally characterize new mutants, it is desirable to produce highly-purified enzyme from expression systems. The procedure, which included PEI treatment, BioRex-70 chromatography, and Heparin-Agarose chromatography, yielded DNA-free and nuclease-free Klentaq enzyme purified to homogeneity, as judged by a single band in Coomassie stained protein gel (23). The same purification procedure also worked very well for purification of Help in essay writing kalä±plarä±, cold sensitive Klentaq mutants (23). This procedure was readily adaptable to accommodate purification of mutant polymerases that display unusual features such as changed affinity and elution profile on money back a particular chromatography resin.

The efficiency of a compare on dogs, each step in the purification scheme was monitored easily by a standard DNA incorporation assay. [0124] The amplification activity of the Essay money back valuation, obtained mutant enzymes were extensively evaluated in Critically thinking in writing your PCR amplification of various gene targets. The new enzymes were tested both in Essay money back conventional and real-time PCR with SYBR green fluorescent detection. These tests included at Do my for me, least about 20% whole human blood (untreated, EDTA-treated, or heparinized), or blood IgG and hemoglobin fractions equivalent. Optionally, the differential sensitivities that the polymerase mutants display toward whole blood were evaluated by performing an amplification activity titration experiment with increasing incremental amounts of Essay, whole blood added to Resume assistance letter, the assay mixtures from about 5% whole blood (vol/vol) to back, about 25% whole blood (vol/vol). [0125] Figure 1 illustrates the results of screening of papers, a collection of 40 KT mutants by PCR assay with homogeneous PCR assay solutions containing 10% whole blood (vol/vol), wherein the Klentaq gene represented the target nucleic acid. Back Valuation! The primers used in Do my for me the PCR assays comprise KT1 (SEQ ID NO:l 1) and RevTaqH (SEQ ID NO: 12), which resulted in the specific amplification of money valuation, a 1.65kbp target fragment. [0126] Figure 2A illustrates the to write, results of typical PCR assays with homogeneous PCR assay solutions containing different amounts of whole blood (vol/vol) in the reaction, wherein an Essay back valuation endogenous human gene from blood represents the target nucleic acid. Critically Thinking! The primers used in the PCR assays comprise DMDex21f (SEQ ID NO:13) and DMDex21r (SEQ ID NO:14), which resulted in the specific amplification of a 0.32kbp target fragment of the endogenous human Duchenne muscular dystrophy gene (Dystrophin). [0127] In order to confirm the blood resistance feature of the Essay money, Klentaq mutant enzymes, numerous exogenous and Do my, endogenous test gene targets were used. Two- three ng plasmid pWB254 DNA or human DNA were used as exogenous targets to amplify the Klentaq gene itself (1.65 kb fragment, which was obtained with primers KT1 (SEQ ID NO: 11) and RevTaqH (SEQ ID NO: 12)) or a 4.3 kb fragment of the human TPA gene (obtained with primers TPA forward (SEQ ID NO: 17) and TPA reverse (SEQ ID NO: 18)), respectively. The endogenous targets (from DNA present in the blood cells) included a 0.32 kb amplicon of the human Dystrophin gene (obtained with primers DMDex21f (SEQ ID NO: 13) and DMDex21r (SEQ ID NO:14) and 1.1 kb or 2.5 kb amplicons of the human CCR5 gene (obtained with primer pairs ccr5+lkb (SEQ ID NO:9)/CCR5-KOZ (SEQ ID NO:7) and Essay back, CCR5-2kb (SEQ ID NO:8)/ccr5deltaRT (SEQ ID NO: 10), respectively).

Whole or EDTA-treated (4.8 mM EDTA) human blood was added at concentrations 0%-20% to the PCR cocktail prior to PCR (homogeneous PCR setup). Things A Compare And Contrast Essay On Dogs! As illustrated in Essay back valuation Figs. 2B and 2C, KT-10 and KT-12 mutants easily amplified the targets in at least 20% whole blood. The wild-type Taq enzyme failed under comparable conditions. The amplification signal obtained with the mutants when detecting endogenous blood genes was gene-dose-responsive. [0128] Example 2. Full-length Taq DNA polymerase mutants display blood- resistant activity. [0129] Importantly, the reading and writing, amino acid changes responsible for money back valuation the blood-resistant phenotype of the Klentaq, were also sufficient to writing cover letter, render the Essay money valuation, full-length Taq blood- resistant when these amino acid changes were incorporated into Things to write on dogs, the full-length gene. For example, the money back, amino acid changes of Help writing kalä±plarä±, KT-10 and KT-12 mutants were incorporated into the full-length Taq gene to generate the analogous Taq-mutants FL-10 and money, FL-12. Find! As shown in Figure 3A (for FL-10) and Figure 3B (for FL-12), both full-length Taq mutants exhibited very high resistance to Essay, blood inhibition, and successfully amplified the endogenous human Dystrophin and CCR5 genes in homogeneous PCR solutions containing 20% blood.

The observed high blood resistance of these mutants reflects dramatic change in the property of the Taq enzyme, considering the fact that the wild-type Taq is typically inactivated in homogeneous PCR assay solutions containing as little as 0.1-0.5 % whole blood. Various commercial Taq enzymes, including AmpliTaq Gold ® , JumpStart™ Taq, and Ex Taq™ failed to detect endogenous blood genes even at the lowest blood concentrations tested. One su?rising exception was the enzyme Z-TAQ™, which showed a significant blood resistance at Help in essay writing kalä±plarä±, 5% and back valuation, 10% blood; however, the and writing, FL-12 polymerase mutant outperformed Z-TAQ™ when used in homogeneous PCR assay solutions containing 20% blood to amplify a 1.1 kbp fragment of the endogenous CCR5 gene with primers ccr5+lkb (SEQ ID NO:9) and CCR5-KOZ (SEQ ID NO:7)) (Fig. 3B). The molecular change in the Z-TAQ™ enzyme responsible for its blood-resistant property is Essay back valuation, unknown, as the manufacturer (Takara) maintains its composition as a proprietary secret. [0130] Example 3. Mutagenized Klentaq mutants with a faster DNA elongation rate. [0131] The screening factor here is to to write on dogs, simply shorten the DNA extension step of the money valuation, PCR cycle beyond the past, point where the wild-type or prior art enzyme stops working. In the case when wild-type Klentaq amplified its own gene, the amplification efficiency was significantly lower at 60 seconds extension step (Fig.

4A, lane 1 at 1 min). Back! Additional tests with discrete extension times showed that the Help kalä±plarä±, Klentaq polymerase did not display amplification activity in PCR assays performed under conditions that employ an extension time of about 50 sec or less (e.g., see Fig. 4A, lane 1 at 30 sec and 20 sec). On the valuation, other hand, mutant Klentaq clone KT-7 displayed amplification activity with the assistance letter, same target in PCR assays under conditions having an money extension step of as little as about 12 sec. (Fig. 4B, lower panel). For the evaluation of Resume assistance with, fast-elongating mutants, extension times in the PCR cycle not exceeding 20 sec per 2 kb amplicon were used. The KT mutants, KT-7 (SEQ ID NO:6), KT-11 (SEQ ID NO:21), and KT-12 (SEQ ID NO:24) were markedly faster elongating polymerases than KT-1 (SEQ ID NO:2), whereas the full-length Taq mutant, FL-12 (SEQ ID NQ:30), displayed increased elongation activity relative to Z-TAQ™ (Fig.

4C). For these experiments, the PCR assays were conducted using homogeneous PCR assay solutions with KT1 (SEQ ID NO: 11) and RevTaqH (SEQ ID NO: 12), which resulted in back valuation the specific amplification of a 1.65kbp target fragment from the Klentaql gene. [0132] Example 4. Hot start achieved by underlay of heavy liquid component can enhance yield of specific amplification products — heavy hot start amplification. [0133] This amplification procedure permits one to obtain an enhanced specificity and reliability from a PCR assay. The strategy is also amenable to PCR assays involving whole blood, as described below. In Writing Includes Your! In two preferred embodiments, two heavy hot start mixes are disclosed that differ mainly in the amounts of Mg 2+ and dNTPs present in the reaction mixture, since the optimum Mg 2+ and dNTP concentrations for Klentaql and KlentaqLA is higher than for Essay money Taq and TaqLA.

These heavy hot start mixes can be stored for at least a month at 4° C. [0134] 10 ? TCA is 500 mM Tris-HCl pH 9.2, 160 mM ammonium sulfate. When the pH of the Tris-HCl stock was adjusted to pH 9.2, the Do my homework for me assignments, pH of the money valuation, aliquots was measured at a buffer concentration of 50 mM in water at room temperature. In Writing Your! The concentration of the money back, 1 M MgCl 2 stock was confirmed by determining the refractive index of the solution using a refractometer and by reference to Refractive Index- Concentration Data in a technical manual, such as THE HANDBOOK OF CHEMISTRY AND PHYSICS by Chemical Rubber Company. [0135] The heavy mix recipe for the KlentaqLA yielded a final Mg(II) cation concentration that was 2.5 mM greater than the total concentration of the dNTP. This heavy mix recipe consists of the thinking includes, following components: 100 ?l of Essay back, 10* TCA; 100 ?l of a dNTP mix consisting of 10 mM dATP, 10 mM dGTP, 10 mM dCTP, and Critically thinking your, 10 mM dTTP; 140 ?l of 100 mM MgCl 2 , 67 ?l of 0.75 mM Cresol Red, 4.25 mM Tris Base, 400 ?l of 50% Sucrose or Sorbitol; and money valuation, 193 ?l of water to 1 ml. [0136] The heavy mix recipe for Taq or TaqLA yielded final Mg(II) cation concentration that was 0.75 mM greater than the total concentration of the dNTPs. This heavy mix recipe consists of the reading, following components: 100 ?l of 10 ? TCA; 94 ?l of 100 mM MgCl 2 , 16 ?l of 100 mM dATP; 16 ?l of 100 mM dGTP; 16 ?l of 100 mM dCTP; 16 ?l of back, 100 mM dTTP; 67 ?l of 0.75 mM Cresol Red, 4.25 mM Tris Base 400 ?l of How to motivation a paper, 50% Sucrose or Sorbitol; and 275 ?l of water to back valuation, 1 ml. [0137] Typical reaction mixtures were assembled with the following components: 3.75 ?l 10* TCA; 1.0 ng target DNA; 1.0 ?l (each) 10 ?M primers; 0.25 to Resume assistance with letter, 0.50 ?l enzyme; 30.25 ?l water to a final volume of 37.5 ?l. This initial mixture represented the top layer. The top layer was added to the PCR assay tube, followed by the addition of oil (if desired or necessary). Money Back! The PCR tube was subjected to a brief centrifugation step to Things a compare, resolve the aqueous and oil layers. Finally, 13.0 ?l of heavy mix was added as an underlayer of the PCR tube contents without mixing.

The tubes were closed and carefully carried to and installed into money back, the thermal cycler without undue agitation. The thermal cycler was set to start with a 5 min heating step from 60° C to 68° C before the first heat denaturation step. A visual inspection of the tubes thereafter confirmed that the Resume writing assistance cover letter, two layers had already mixed during this time. [0138] For heavy hot start PCR assays that included whole blood in the heavy layer, the following experiment was performed. One hundred microliter reactions were assembled with the whole blood being added last. The top layer consisted of 80 ?l mixtures, wherein each mixture contained 0.25 ?l of polymerase selected from the group consisting of Klentaql (Klentaq-278), Klentaq5 (Klentaq-235), Klentaq?, Klentaq7, additional mutants, and Taq. Before the blood was added, water was added to money, complement the blood volume, so that at the final volume would be 100 ?l, even though the Critically thinking includes, volume of the heavy, whole blood underlay ranged from 0.5 ?l to 20 ?l. The blood was carefully added at the bottom of the tubes, underneath the 80 ?l top layer. For example, in PCR assays that contained 0.5 ?l of blood, 19.5 ?l of Essay, water was added to the upper layer before the blood was added as an Do my homework homework make underlay at money, the bottom of the tube. English Reading And Writing Past Papers! The layers were not manually mixed before the money back valuation, PCR assay was performed. The primers were present at Things and contrast, 20 pmoles each per 100 ?l reaction.

The buffer was KLA pH 9, the concentration of dNTPs was 100 ?M each, and 1.3 M betaine was present (all concentrations as final in Essay the 100 ?l). Ten nanograms of English reading and writing, human DNA (from Novagen) was included in the two of the no-blood reactions (the ones catalyzed by Klentaq-235 and back, Taq) (indicated by lanes denoted by 0+) to provide a positive control for in essay the polymerase activity. The thermal cycling program was 3 min preheat at 60° C, 35 cycles of (71 sec at 93° C, 60 sec at 60° C, and 5 min at 68° C). [0139] Figure 5A illustrates the results of Essay back, heavy hot start PCR assays (100 ?l reaction volumes) conducted with KT-1 (SEQ ID NO:2), KT-6 (SEQ ID NO:4) and KT-7 (SEQ ID NO:6) in the presence of whole blood and find motivation to write research, under different conditions of money back, pre-treatment of the writing with cover letter, reaction samples prior to initiating the thermal cycling program. The asterisks indicate those reaction vessels wherein the Essay money, heavy and light volume component layers were premixed by vortexing, i.e., reactions that a contain homogeneous PCR assay solution and that were not subjected to a heavy hot start procedure as described herein. Lanes 1-13, 15 and 17 are PCR assays directed toward the writing cover letter, amplification of a 1.1 kbp target from the human CCR5 gene using ccr5+lkb (SEQ ID NO:9) and CCR5-KOZ (SEQ ID NO:7). Lanes 14, 16, and 18 are PCR assays directed toward the amplification of a 2.5kbp target from the money valuation, human CCR5 gene using CCR5-2kb (SEQ ID NO:8) and ccr5deltaRT (SEQ ID NO: 10). [0140] Figure 6 depicts the additional results of this type of experiment. The amplification activity was revealed by Things a compare on dogs the specific amplification of a 0.5 kbp DNA product from the CCR5 gene endogenous to the human cells in the blood (except for the lanes indicated by 0+, which indicates the presence 10 ng of exogenous human DNA template without whole blood). KT-1 (SEQ ID NO:2), KT-6 (SEQ ID NO:4), and KT-7 (SEQ ID NO:6) displayed DNA amplification activity in reaction containing from about 1% whole blood (vol/vol) to about 20% whole blood (vol/vol) whereas Klentaq5 and Taq did not display amplification activity in reactions containing as little as about 1% whole blood (vol/vol). The primers used to generate this amplification product were CCR5-D5 (SEQ ID NO: 15) and CCR5-D3 (SEQ ID NO: 16). Essay Money Back Valuation! [0141] Example 5. Whole blood PCR assays that employ KT mutant polymerases with a second thermostable DNA polymerase having a.

3 '-exonuclease activity. [0142] This example shows that long and accurate PCR works with whole blood as the thinking in writing, source of the target template. Since long and accurate PCR (US Patent. 5,436,149, claims 6-16) comprises the use of a mixture of DNA polymerases, this example also illustrates that the Essay money valuation, minor component of the mixture, an archaebacterial DNA polymerase which is thermostable and Resume assistance cover, which exhibits 3'- exonuclease activity, is su?risingly active with whole blood. [0143] The master PCR cocktail was assembled as follows: [0144] 200 ?l l0x KLA pH 9. [0145] 20 ?l 10/40 (mix of 10 mM each dNTP and 40 mM MgCl 2 ) [0146] 520 ?l 5 M Betaine. [0147] 40 ?l primer CCR5-2kb (SEQ ID NO:8) [0148] 40 ?l primer ccr5deltaRT (SEQ ID NO: 10) [0149] 1120 ?l water to make 20 x 97 ?l reaction mixture aliquots. [0150] 1940 ?l total cocktail volume. [0151] It is Essay money back, worth noting that the PCR cocktail lacked target nucleic acid template and the DNA polymerase at this stage. Reading And Writing Past Papers! [0152] Enzyme dilutions were prepared on money back ice by mixing them with a portion of the master mix as follows: six aliquots (75 ?l each) of English reading, master mix were withdrawn and added to Essay money valuation, an aliquot (0.75 ?l) of enzymes KT-1(SEQ ID NO:2), KT-6 (SEQ ID NO:4), or KT-7 (SEQ ID NO:6) each at homework homework, about 30 U/?l, and Essay money back valuation, the same three enzymes that have been previously mixed with 1 :24 dilution volume of the archaebacterial enzyme Deep Vent, which is available commercially at find motivation to write a paper, 2 U/?l. These latter enzyme mixtures possessed a ratio of back valuation, KT enzyme to Deep Vent enzyme of about 1 :360. [0153] Aliquots of the Do my for me homework, master mix (72 ?l) were dispensed to reaction tubes, then aliquots of the appropriate enzyme dilution mix (25 ?l) were dispensed into the reaction tubes to provide for Essay money back a total volume of 97 ?l. [0154] Pure human DNA (Novagen), stored at a temperature of to write and contrast, 4° C and at a concentration of 3 ng/?l, was diluted 3-fold with standard TEN buffer (10 mM Tris pH 7.9, 10 mM NaCl, 0.1 mM EDTA) to make 1 ng/?l, and then an aliquot of this solution (3 ?l) was pipetted into the aforementioned 97 ?l mixture to yield the final PCR assay mastermix. Money Valuation! [0155] Whole blood, which is typically stored in an aliquot of 0.5 ml with 4.5 mM EDTA at -80° C, was thawed at room temperature for about 15 to to write, 30 minutes and back, mixed by How to to write gentle inversion before 3 ?l was pipetted underneath the aforementioned 97 ?l mixture in additional PCR reaction tubes, avoiding mixing. The pipettor was set to 3.2 ?l, and care was exercised not eject the last small amount of blood volume (-0.2 ?l), so as to avoid injecting a bubble of air into the PCR assay solution and thereby disturb the valuation, heavy phase at the bottom of the tube.

[0156] Thermal cycling for the PCR amplification was carried out Critically, using a similar program as described above (2 minutes at 93° C, followed by 33 cycles of (71 seconds at Essay back valuation, 93° C, 1 minute at 60° C, 10 minutes at 68° C). Writing Assistance! After the PCR assays were completed, aliquots of the reactions (18 ?l) were mixed with 4.4 ?l of blue dye mix, and analyzed by Essay money back electrophoresis on Do my assignments a 1.4% agarose gel. [0157] Figure 7 illustrates that little or no PCR product of the expected size (2.5 kbp) is obtained unless an amount of Deep Vent polymerase is Essay, present to complement the major DNA polymerase Klentaql (SEQ ID NO:2), Klentaq? (SEQ ID NO:4), or Klentaq7 (SEQ ID NO:6), all of and writing, which perform equally well under these conditions. [0158] Prophetic Example. Selection of blood-resistant Klentaq DNA polymerase mutants using compartmentalized self-replication. [0159] The recently described highly effective compartmentalized self-replication (CSR) strategy for directed evolution of enzymes (25, 26) could be adapted to select for blood-resistant Klentaq mutants. Back! The existence of in writing, blood-resistant mutant(s) of Klentaq should be evident in the library as a manifestation of detectable self-replication of the Essay money, Klentaq gene in Resume with the presence of 10% blood, a concentration that is inhibitory for the wild-type Klentaq.

Blood-resistant Klentaq clones could be isolated and back, Klentaq mutant proteins prepared according to with cover, the procedures set forth in Example 1. Individual Klentaq polymerase mutants could then subjected to screening procedures to ascertain whether each displays a cold sensitive phenotype. Those Klentaq mutants that are blood-resistant and display a cold sensitive phenotype would be expected to conform to the group of Essay valuation, blood- resistant DNA polymerases as defined herein. The aforementioned selection/screening procedure should also be amenable to identifying full-length Taq DNA polymerase mutants that are blood-resistant DNA polymerases as defined herein. Sequence Information [0160] The nucleic acids and polypeptides of the various DNA polymerases and the oligonucleotide primers described in with letter this application include the sequences shown in money valuation the Sequence Listing. Table II provides the nucleic acid sequences for the specific oligonucleotide primers used in the various examples disclosed in this application.

PCR-inhibitory components in blood cells. J. Clin. Microbiol. Do My For Me Homework! 39:485-93. 8. Frackman S, Kobs G, Simpson D, Stores D. 1998. Betaine and Essay, DMSO: enhancing agents for PCR. Promega Notes 65:27.

9. Topal MD, SinhaNK. 1983. Products of bacteriophage T4 genes 32 and 45 improve the accuracy of Things on dogs, DNA replication in Essay back valuation vitro. J. Biol. Chem. 258:12274- 79. 10. Reading And Writing Past! Akane A, Matsubara K, Nakamura H, Takahashi S, Kimura K. 1994.

Identification of the Essay back, heme compound copurified with deoxyribonucleic acid (DNA) from in essay, bloodstains, a major inhibitor of Essay money, polymerase chain reaction (PCR) amplification. J. Forensic Sci. 39:362-72. Resume Cover Letter! 1 1. Kreader CA. 1996. Money! Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein.

Appl. Environ. Microbiol. 62:1102- 06. 12. Morata P, Queipo-Ortuno I, Colmenero J. In Essay Writing! 1998. Strategy for optimizing DNA amplification in Essay money back a peripheral blood PCR assay used for diagnosis of thinking includes, human brucellosis. J. Clin. Microbiol. 36: 2443-46.

13. Rossen L, Noskov P, Holmstrom K, Rasmussen OF. 1992. Inhibition of PCR by Essay money back components of food samples, microbial diagnostic assays and DNA- extraction solution. Homework For Me Homework Assignments Make! Int. J. Food Microbiol. Essay Back! 17:37-45. 14.

Izraeli S, Pfleiderer C, Lion T. 1991. Detection of in essay writing, gene expression by. PCR amplification of RNA derived from frozen heparinized whole blood. Nucleic Acids Res. 19:6051. 15. Essay Back! Wilson IG. 1997. Inhibition and facilitation of nucleic acid amplification. Resume Writing Assistance With Cover Letter! Appl. Environ.

Microbiol. 63:3741-51. 16. Al-Soud AW, Lantz P-G, Backman A, Olcen P, Radstrom P. 1998. A sample preparation method which facilitates detection of bacteria in blood cultures by the polymerase chain reaction. J. Microbiol. Methods 32:217-224. 17. Klein A, Barsuk R, Dagan S, Nusbaum O, Shouval D, Galun E..

1997. Valuation! Comparison of methods for extraction of nucleic acid from writing, hemolytic serum for PCR amplification of hepatitis B virus DNA sequences. J. Clin. Microbiol. 35:1897-99. 18. Cattaneo C, Graig OE, James NT, Bolton H. 1997. Money Back Valuation! Comparison of three DNA extraction methods on bone and blood stains up to to write, 43 years old and amplification of three different gene sequences. Money Back Valuation! J. Forensic Sci.

42:1 126-35. Find To Write Research! 19. Essay Money Valuation! Bourke MT, Scherczinger CA, Ladd C, Lee HC. 1999. NaOH treatment to Do my for me homework make, neutralize inhibitors of Taq polymerase. J. Essay! Forensic Sci. 44:1046-50. 20. Kox LF, Rhienthong D, Miranda AM, Udomsantisuk N, Ellis K, van Leeuwven J, van Heusden S, Kuijper S, Kolk AH. 1994. A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples.

J. Clin. Help Writing Kalä±plarä±! Microbiol. 32:672-80. Money Back Valuation! 21. Things A Compare And Contrast Essay! Kramvis A, Bukovzer S, Kew MC. 1996.

Comparison of hepatitis B virus DNA extractions from serum by the QIAamp blood kit, Genereleaser, and the phenol-chloroform method. J. Clin. Microbiol. 34:2731-33. 22. Barnes WM. 1992. The fidelity of taq polymerase catalyzing PCR is improved by an N-terminal deletion.

Gene 112:29-35. 23. Back! Kermekchiev MB, Tzekov A, Barnes WM. 2003. Cold-sensitive mutants of Taq DNA polymerase provide a hot start PCR. Writing Kalä±plarä±! Nucleic Acids Res. 31:6139-47. Money Back! 24. Tabor S, Richardson CC. 1995. A single residue in DNA polymerases of the E. coli DNA polymerase I family is critical for in writing includes distinguishing between deoxy- and dideoxyribonucleotides.

Proc. Natl. Acad. Sci., USA 92:6339-43. 25. Tawfik DS, Griffiths AD. Essay Money Back Valuation! 1998. Man-made cell-like compartments for molecular evolution. Nature Biotech. 16:652-56. 26.

Ghadessy FJ, Ong JL, Holliger P. Motivation To Write A Paper! 2001. Direct evolution of polymerase function by compartmentalized self-replication. Proc. Natl. Acad. Valuation! Sci., USA. 98:4552-57. 27. In Essay Kalä±plarä±! Barnes WM. 1994. PCR amplification of up to 35 kb DNA with high fidelity and high yield from bacteriophage templates.

Proc. Essay Valuation! Natl. Acad. Sci., USA 91:2216-20. 28. Resume Assistance Letter! Barnes WM. 1994. Tips and tricks for long and accurate PCR. TIBS. 19:342-46. 29. Ausubel FM, Brent R, Kingston RE, Moore DD et al. 1987.

CURRENT PROTOCOLS IN MOLECULAR BIOLOGY. John Wiley Sons, New York. 30. Sambrook J. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor. 31. Barnes WM. Valuation! July 25, 1995. Find Motivation To Write A Paper! U.S. Patent No.

5,436,149, Thermostable DNA polymerase with enhanced thermostability and enhanced length and money back, efficiency of primer extension. 32. Barnes WM. April 1, 1997. To Write A Compare Essay On Dogs! U.S. Patent No. 5,616,494, Thermus aquaticus DNA polymerase lacking the n-terminal 235 amino acids of Essay valuation, taq DNA polymerase.

33. Scalice ER, Sharkey DJ, Daiss JL. 1994. Monoclonal antibodies prepared against the DNA polymerase from Thermus aquaticus are potent inhibitors of enzyme activity. J. Thinking In Writing Includes! Immunol. Methods 172:147-63. Essay Valuation! 34. Sharkey DJ, Scalice ER, Christy KG Jr, Atwood SM, Daiss JL. 1994.

Antibodies as thermolabile switches: high temperature triggering for the polymerase chain reaction. Things To Write Essay! Biotechnology 12:506-9. 35. Kellogg DE, Rybalkin I, Chen S, Mukhamedova N, Vlasik T, Siebert. PD, Chenchik A. 1994. TaqStart Antibody: hot start PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. Biotechniques 16:1134-7. Back Valuation! 36. Baskaran N, Kandpal RP, Bhargava AK, Glynn MW, Bale A, Weissman SM. 1996. Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.

Genome Res. Thinking Your! 6:633-8.

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Top 10 Details to Include on a Nursing Resume. As healthcare recruiters, we reviewed thousands of nursing resumes. Unfortunately, the Essay, vast majority of Help in essay writing kalä±plarä± them were missing multiple details that were important to the Nurse Managers and Nurse Supervisors who were largely responsible for making the Essay money valuation, hiring decisions. Moreover, missing these critical details can ultimately lead to a lower ranking within the Applicant Tracking Systems so prevalent in today’s hiring process. We hope the English reading papers, information provided here will help you create an amazing resume! First, it’s important to have an idea of how the money valuation, hiring process typically works in order to Help writing thoroughly understand the importance of these critical details.

When a job opens up to the general public, the healthcare employer can sometimes receive hundreds of resumes. In some cases, the resumes are electronically ranked by money, Applicant Tracking Systems that award higher rankings for resumes that include the critical skills and experiences sought for the job. In any case, staffing office representatives, mostly recruiters, will review the rankings and resumes and pass along the “best” candidates to the hiring managers. It’s important to note that being the “best” often means having a resume with all the critical details. If the information isn’t there, then the Help in essay writing, resume stands a far greater chance of being removed from the process. It’s important to note that missing these details is not the nurse’s fault.

Unfortunately, the back valuation, vast majority of informational resources pertaining to resumes is very general in and writing past nature. Essay. General resources are useful only for people with very general careers like “Sales”. These resources are mostly useless for nurses. Meanwhile, many resources specifically related to nurse resumes offer insufficient detail. Moreover, healthcare employers do a terrible job at conveying what they’re looking for in their job descriptions, so candidates have no clue what to include in their resumes. Skimming the Things to write, list below may leave the impression that everything on back valuation, it is Help in essay writing kalä±plarä±, obvious.

However, reading the details provided for each item will most likely uncover items which are missing from your nursing resume . What to money back valuation Include on Your Nursing Resume. There are literally thousands of potential professional affiliations for nurses. In most cases, employers want to know if you belong to any. For example, if you belong to the American Association of Critical Care Nurses or the Emergency Nurses Association, then most employers would like to know this. Include the a compare and contrast essay, following information: Affiliation name Your date of valuation admission Offices held Brief description of your role or reason you chose this group over others. 9. Honors/Awards/Special Assignments: As with every other resume, a nurse’s resume should include any honors and awards they’ve received. Examples include honors and awards from school, work, volunteer work, professional affiliations, and even social clubs. Nurses should also include any special assignments they were given at work. Did you ever take charge duty?

Did you do any scheduling or mentoring? You may choose to place these items under their own heading. However, this isn’t necessary. Things And Contrast Essay. You can also add them where applicable throughout the rest of your resume. 8. Specific Nursing Education Details: Including the degree you earned (ADN, ASN, BSN, MSN, etc.) is a must. Not including it increases the chances that your resume will be removed from back valuation, consideration. This is because the to write, information is often required for the resume to money move through the process and it isn’t easily attainable by thinking in writing your, the staffing office. So, they’ll often pass on a resume that doesn’t include the information in money valuation favor one that does. Provide at least the following information about English reading and writing past, your education: Name of Essay money school Degree earned Beginning date Completion date City, and State.

You might also consider adding a description that includes achievements, awards, scholarly organizations and activities, or your GPA if it was really high. Of course, scholastic achievements are more important the earlier you are in your career, so keep that in mind when crafting the education section of Do my for me your resume. Finally, you may also wish to include details on any continuing education units you’ve taken within the last 2 years. Bonus: Are you bilingual? If so, be sure to include it on your resume! According to money back valuation a recent study from Wanted Analytics, “bilingual” was the second most commonly required skill listed on writing assistance with cover letter, nursing job advertisements. 7. Nursing License and Certification Details: It is highly recommended that you include the Essay money back valuation, following for your licenses: License type (LPN, RN, NP, CRNA, etc.) Licensing State/Body Name on license if different from name on resume License expiration date License number If the on dogs, license is back, part of the Resume writing cover, Nurse Licensure Compact, then state it clearly.

Many people argue that a nursing license number should not be included on a resume as a matter of privacy. Money Valuation. However, nursing license numbers are a matter of public record. They can be easily obtained from each state’s Board of Nursing using the standard information included on your resume. Providing the license number simply makes the recruiter’s job easier. And in a competitive job market, that could make all the difference in the world. When it comes to certifications, it is highly recommended that you include the following for every certification you hold: Certification name (BLS, ACLS, PALS, TNCC, etc.) Certifying body (AHA, etc) Expiration date, or date acquired if it has no official expiration date.

Unlike most professions, nursing is conducted round-the-clock. Therefore, it’s best to convey the shifts you’re willing to work on your nursing resume. Are you open to How to find motivation to write a paper working 8, 10, and/or 12 hour shifts? Are you open to working Days, Mids, PMs, and/or Nocs? You should include this information even if you’re applying for a specific job with a specific shift. Essay Money Valuation. You never know if the employer has another opening that is Do my homework assignments, unadvertised which you maybe qualified for. Moreover, resumes aren’t always submitted to specific job advertisements.

In fact, many sources indicate that 80% of all jobs are filled through networking. Including your availability is very helpful for valuation general job inquiries. In addition, you should indicate your willingness to and writing past relocate when applicable. These statements regarding availability can be given their own heading, but it’s best to simply add them to Essay money valuation your Professional Summary. The healthcare industry is going electronic. Paper charting will soon be a way of the past. It’s imperative to list any and all Electronic Health Record (EHR) and Electronic Medical Record (EMR) experience you have. According to a recent study by Wanted Analytics, “Electronic Medical Record” was the most commonly required skill for nurses listed in Do my for me homework make nursing job advertisements. “Epic Software” and money, “Meditech”, popular EMR software packages, were also on the list. Of course, billing codes are a big part of EMRs. And both ICD10 and ICD 9 are among the Critically thinking your, most commonly listed skills in job advertisements. Valuation. So if your scope of practice deals with this in any way, then be sure to add these to your resume.

You may also want to Do my homework assignments include any other computer experience you have just in case it may be of value to the employer, or to at least demonstrate that you have computer skills if you lack experience with EHRs and EMRs. Signifying the type of facility you worked in tells the potential employer a ton about your experience with very few words. You should know the exact designation of all the facilities you worked with and Essay back, if you don’t, now is the time to find out. Short term acute care? Long Term Care?

Long Term Acute Care? Senior Nursing Facility? In addition, if you worked at a Trauma Hospital, then you should include this on your resume along with the trauma designation (1, 2, 3, etc). If you worked at a teaching hospital, then you should include this information. Such information can be included under the specific job description in your resume’s Work History section, or in your resume’s Summary.

For example, if the job listing you’re applying for lists trauma hospital experience as necessary or desired, then it’s a good idea to Critically thinking your identify your trauma experience in the resume Summary to make it stand out. Include the number of total beds at the facilities you worked at, as well as the number of beds in the specific units on valuation, which you worked. Just like the facility type, providing the number of beds tells the potential employer a ton about your experience with very few words. We regularly saw resumes that listed things like “3 West” as the unit. Unfortunately, only people who work at that facility know what that means. Instead, include the type of find motivation to write a paper research unit (MS, TELE, ICU, CVICU, ER, LD, etc) in order to Essay money back valuation convey the message. We can’t stress enough how important this is. Also include your caseload. What was the nurse to patient ratio in this unit, and how much support was there in the form of How to find a paper LPNs, CNAs, or MAs? Include any other unit specific details as well. Did the unit take trauma patients?

Did the unit routinely deal with overflow from back, other units? If so, what types of patients were seen from overflow? 1. Specific Details/Duties and Accomplishments Regarding Your Nursing Experience: Up to this point, the focus has been on incidental, although very important, details. English Reading. But what about the back valuation, meat of your resume, the job descriptions and experiences that make up the bulk of your resume? When it comes to this, nurses are in the precarious position of balancing duties with accomplishments. You see, the Do my make, current standard recommendation for resumes is to make them accomplishment driven. The idea is that you should provide specific accomplishments, as opposed to duties, that illustrate how your work translated into quantifiable and tangible results for money valuation your current and previous employers. Advocates of this approach advise against listing duties on your resume. However, healthcare employers need to know that you have experience with the highly technical duties that are integral to the job you’re applying for.

At the same time, they too want to know about your accomplishments. English Reading Papers. Therefore, it’s important to include details about the specific day-to-day duties you performed. However, at the same time, you don’t want to fall into the trap of creating a “duties-driven resume”. Managing this challenge represents a key difference between nursing resumes and general resumes. Before we continue, it’s best to address the 800 pound gorilla in this conversation.

Nurses sometimes have hundreds of duties and responsibilities. How do you choose which ones to list because you certainly can’t list them all? We hate to say it, but including things like, “Provided patient care” is a complete waste of space. It’s simply too general. Our experience indicates that many nurses believe that nursing is very similar no matter where you go which may be the reason that such general statements are so often included in nursing resumes. In reality, the differences are stark between various facilities.

For example, the Step-Down Unit at one hospital may not work with Swan-Ganz Catheters while the SDU in another hospital may. With that in mind, here are some general themes to consider when determining the money back valuation, types of Help in essay duties to Essay money include on How to find motivation, your resume. This list is Essay back, not comprehensive, but it should help you understand the level of English papers specificity that we’re talking about: Did you start IVs? Did you administer medications? Which medications? What type of patients did you care for? Renal? Cardiovascular? Neuro? Ortho?

Rehab? What was the age range of the patient population you cared for? What specific equipment do you have experience with? Did you read strips? Work with vents? Trachs? Balloon pumps? Swan-Ganz? da Vinci Surgical System?

Which industry-wide protocols, processes and procedures are you experienced with? For example, AIDET is among the most commonly listed requirements in nursing job advertisements. There are many similar protocols, processes and procedures throughout the industry, so be sure to make note of money valuation those that were used by your previous employers. Now, we’re still in the tough spot of determining exactly which skills and duties to include on the resume. In Writing Includes. First, start by Essay back valuation, including any duties specifically mentioned in the job description that you have experience with. Next, do some research on the employer in assistance with cover letter question to find specific details that may help you decide which duties might be important to list. Essay Valuation. Use the company website, news, and any professional connections you have in an effort to determine the Help, types of Essay money patients, processes and procedures common to assistance cover letter the specific employer in question. Once you have the money valuation, duties narrowed down, there are a couple of How to to write a paper ways to convey them on your nursing resume. First, you can incorporate the duties into Essay money back, your “accomplishment statements”. Letter. We discuss this approach below. Second, you can simply list out the duties.

While this is less preferred, it is sometimes not possible to accomplish any other way. Money Back. Your summary is a good place for this. And Writing Past Papers. For example, you might include the money back valuation, following in your resume summary if you’re applying for a position in the CVICU: Proficient with starting IVs, Intra-aortic balloon pumps, 12-Lead Placements, 12-Lead Interpretation, AICD Insertion, Beta Blockers, Argotroban, Atropine and other cardiovascular medications. Bonus: Given the large number of skills and duties nurses are responsible for, you may consider utilizing a “Skills Checklist” during your job search. Skills Checklists are self assessment tools commonly used throughout the reading and writing past papers, healthcare industry. BluePipes provides members with free access to over 100 comprehensive skills checklists that can be completed, saved and downloaded as PDF documents. You can view a sample here. They’re a great way to convey your skills to potential employers.

You might consider uploading them along with your resume when applying for jobs or you can bring them to your job interviews. Considerations for Accomplishments. Now that we’ve covered duties, let’s take a look at accomplishments. First, you can try to frame duties inside “accomplishment statements” in order to knock them both out at the same time. Essay. In other words, offer an explanation about how you achieved results while performing your duties.

Again, this isn’t always possible, but here are some considerations: Did you receive recognition or awards from your previous/current employer? Did your previous/current employer receive recognition or rewards? How did performing your duties contribute to that? When framing accomplishments, it’s also useful to know how your previous and English papers, current employers quantified their success as an organization. Essay Money Back. For example, patient satisfaction is typically tracked with programs like HCAHPS or Press Ganey. Did your employer experience improvements with such indicators?

How did performing your duties influence that? Next, it’s important to understand how your current and Critically thinking includes, former employers measured your individual performance as this is Essay back valuation, very helpful when framing accomplishments. Nearly every healthcare employer conducts employee evaluations. Evaluations typically offer both qualitative and quantitative information that can be leveraged when framing accomplishment statements. Again, you may choose to list specific duties you’re proficient with separate from your accomplishments. Or, you may choose to frame the duties within your accomplishment statements. Or you may choose a combination of the two approaches. In any case, it’s important to provide both job-specific duties and accomplishments on your nursing resume.

Bonus: Healthcare is very diverse. Many hiring managers would also like to research know what other skill sets you have outside of Essay back your primary area of Help writing expertise. Did you float to the ER or LD units when needed? If so, then you may want to Essay valuation provide some reference to the skills and accomplishments you achieved in these areas. General perspective on nursing resumes.

This may seem like a ton of information to incorporate into a standard resume. However, nursing is not a standard profession and concerns over resume length are becoming antiquated with the advent of Applicant Tracking Systems. Moreover, the and writing past, push to force nursing and healthcare resumes to conform to Essay money back valuation the standard format that serves general professionals, like salespeople, is a disservice to both healthcare professionals and employers. Healthcare professionals often miss opportunities to highlight skills and experience that are highly sought after. As a result, healthcare employers often miss out on perfect candidates.

This push toward generalized conformity is Things essay on dogs, even prevalent on the most popular job boards, like Monster and CareerBuilder, and Essay, professional networking services like LinkedIn. Things Essay. Nurses and other healthcare professionals are better served by industry specific professional networking services like BluePipes. For example, BluePipes provides nurses with a profile builder capable of recording healthcare specific career details as well as the ability to print the Essay money back, profile to English reading past PDF as a resume formatted specifically for healthcare professionals. So it’s like a cloud based resume service. It also offers several other tools that help nurses manage their careers more effectively and efficiently. As always, your feedback is greatly appreciated. Please let us know what you think by Essay money back, posting a comment!

16 Tips To Prepare Your Nursing Resume For Applicant Tracking Systems We’ve spoken to How to find to write a paper countless nurses and other healthcare professionals who. Back Valuation. How to Write the Best Nursing Cover Letter A well crafted nursing cover letter can be just as. Nursing Resume Objective or Summary? Nurses often wonder if they should include an objective or. I am wondering how to list my experience as a maternal-child float RN of 2+ years. I worked 4 different units, all quite different, ranging from mom baby, to peds, peds ed/uc, and nicu.

How do i go about addressing those different areas and How to find motivation to write research, responsibilities and skills, when they all fell under 1 position? Thank you for the information, its very helpful. Back. This site is Resume assistance, of so much help to me. Essay Money. thank you, and it is also free. Homework For Me Homework Make. Its amazing, I just can’t appreciate it enough. I was wondering, i am a one year experience nurse at a step down ICU (PCU), I am now looking for a job in an ICU. The locations and facility i am looking at have nothing for Essay back valuation experience nurses, but have a lot of positions for new nurses that want to work in ICU. I just want to apply to the new nurse position and Help kalä±plarä±, i really don’t mind the money valuation, pay. Now my question is, should i just use my new grad resume with my clinical experience or i should update my resume with my one year experience and a compare essay, apply although the position is for valuation new grads. please i will really appreciate any advise. thanks you. How can I include my Medical-Surgical Certification from ANCC on my resume.

Can I include it on my credentials area on top of my resume – following my name and degree? I ask because the ANCC instructs to do so on their website. I’ll be honest it’s somewhat confusing though, at least to me. Papers. I plan to include my Medsurg certification in certification section of Essay valuation my resume where BLS and English reading and writing past, RN licensure are, etc. I just wanted it to stand out and pop so to back speak – as to not have to writing assistance cover letter read on to notice it. On ANCC site, on a “How to Display Your Credentials” page they instruct you to include RN- BC (Registered Nurse-Board Certified). Essay Money Back Valuation. Was hoping you could clarify or add something else. Great web site … thanks.

Thanks for the inquiry, Jesse, and my sincerest apologies for the delay! Your Medical-Surgical Certification from ANCC is considered a “National Certification” and should be listed after your name at the top of your resume and in the Licenses and Certifications section of your resume. The order for listing credential after your name is: Highest Degree Earned State License National Certification Nationally Recognized Honors and Awards. I hope this helps! I have been working out of the acute care setting in public health nursing for Resume writing with 7 years. I have heard over and over that because I have been out of the acute care setting for so long that I don’t qualify for a lot of the positions that I have applied for.

Admittedly, I have not used many nursing skills for the last 7 years, except for giving immunizations and occasionally drawing blood. I have gotten very weary and feel like I will not be able to valuation find another job, and I am really not happy with my current job. I am unsure of how to make my resume “pop” and have prospective employers want to give me a chance. I am not looking to get back into areas that are highly skilled, but I would not mind getting back into acute care. Any ideas. Kurt, I am an RN at a VA hospital in in writing your cardiac telemetry. One of the biggest hurdles to applying at Essay money back valuation, any VA facility is the fact that USA Jobs is a digital screening tool as much as it is an online application portal. Matching as many keywords in the functional statements for your desired role in your application can be the difference between getting an interview and How to motivation research, being passed over. Hope this helps! In your article you talked about back, whether or not your previous/current employer received recognition or rewards.

I’m working on applying for Help in essay kalä±plarä± a position that is back, open for an orthopedic nurse after quitting my previous orthopedic nurse job. Do My For Me Homework. The hospital that I previously worked received recognition for their total joint replacement program. I was wondering if this would be something that is back, good to include as part of reading and writing past my resume, considering I was a part of the valuation, unit during that time for the hospital’s certification/recognition. If so, how would you go about including that in without making it seem just a random fact listed and make it more as an accomplishment for in essay writing myself also since I was a part of that unit/team during that time. Thanks for the inquiry, Jessica. Yes, this is definitely something you can include on your resume. If you feel that you’re not able to expand in a way that adequately describes your role in the achievement, then you may want to expand in your cover letter. On your resume, you might want to Essay money valuation couple this team achievement with one of your own that demonstrates you were a key contributor. How To Find To Write Research. For example, you might have received an individual award or a high employee evaluation score. You may also want to include this in your summary as opposed to Essay money back valuation the job description to make it stand out a little more. I hope this helps!

I am an experienced OR nurse who has performed in just about every surgery setting from Open heart to Ophthalmology, outpatient as well as pre-op admitting and recovery In the past I have managed a surgery department at a busy hospital and also ran a surgery center. Do My For Me Assignments Make. I have been fortunate to valuation have been able to take off time to kalä±plarä± stay home and be with my children. That being said, I am worried about the years off and blank space on my resume. How would you go about Essay back, filling in the gap? I have volunteered in multiple areas at their schools, from organizing an Emergency Preparedness fair, fundraisers and teaching Compression Only CPR. My license and continuing CEU’s have always rained current. Homework Make. Do you think this will make it difficult for me as I try entering the job setting? I appreciate any suggestions you might have. Thanks for the inquiry.

There is no steadfast rule on valuation, how to handle this situation, but there are two fundamental approaches to consider. First, you can consider a “Functional Resume.” Functional resumes are resumes that focus on skills instead of experiences. In your case, you would focus on all of your OR experience. You might have a small section to list your previous employers. or you may even choose to leave it out entirely. There are many examples of assistance Functional Resumes available on money valuation, the internet. Second, you could use a traditional chronological resume. In this case, you would list your experience raising the kids as one of the entries in your chronological work history. You could include details on the experiences mentioned in How to to write research your comment on this blog post. Again, there is valuation, no right or wrong way.

Each has its advantages and disadvantages. To answer your question, yes, this situation is English reading papers, always a bit of challenge. However, experienced nurses are in high demand at the moment, so you should be fine. Essay Back. Also, I’m personally inclined to recommend the thinking, chronological format. The main advantage of Functional Resumes in money back valuation this particular case is to draw attention to your skills and away from the English and writing past papers, gap in recent experience. However, employers are going to find the gap no matter what. So, I think it’s best to embrace it.

With the chronological resume, you can still include all the applicable skills. I hope this helps! I have worked in home health and corrections for Essay money valuation about 5 years now. I have done a lot of Do my for me assignments make basic nursing ranging from back valuation, blood draws, interpreting labs, starting IVs, wound care, peritoneal dialysis, picc line care (including using clot busters) and removal. IV anti biotics, med passes small and How to motivation research, large including mental health medication passes. I have responded to Essay chest pain calls, shortness of Do my for me homework assignments make breath, hangings, falls, knife wounds, self inflicted wounds, and money valuation, unresponsive patients. I have worked in a corrections hospital with acute and and writing, long term patients as well as hospice patients, but it isn’t seen as ER work even though it seems from money, talking to other nurses that that isn’t so far different than those working in a hospital ER..

Trying to format my resume to English reading past papers make my experience fit into Essay money back valuation, what the Veterans Administration would want/be attracted to.. I am a soon to be retiring Military Nurse and will be transitioning to the civilian workforce. How important are listing awards? I have numerous military awards based on my work ethics and performance on the job, but I am afraid that the general public is not going to English reading and writing past have any idea what they are or mean. Also, any good tips on turning military missions into civilian language? Thanks for Essay money back valuation your service!! I think it’s a great idea to add the awards to your resume. You could do one of two things. With Cover Letter. Simply list them out by their official name. There’s a good chance that the reviewer will search for the award on the internet and find it.

Or, you could add a very brief description of the award in parentheses. For example: X Award (earned for valor in Essay money valuation action). Either way, if you have a lot of awards, then you may want to include only the highest ranking awards. A Compare Essay On Dogs. If you choose to list many of them, then put them in columns or in a continuous stream separated by Essay money valuation, commas to English and writing papers save space. Conveying your military experience in civilian language can be challenging if you did not work in a military hospital. My apologies, but I lack the back valuation, technical expertise to provide detailed recommendations. How To To Write A Paper. That said, I’d recommend reviewing the job descriptions for the jobs you’re applying for valuation use them to guide your efforts whenever possible. Do My Assignments. I hope this helps and thanks again!

I have a position working in a “float pool” (we call it the Mobile Unit). The unit is split into two different tracks, med/surg (HV general acuity patients; ortho/urology/gen/gyn surgery; neuro/ENT; Onc [recent unit merge means I don’t float here anymore]; gen med; adult psych; child/adolescent psych; Postpartum (moms and babies); inpatient rehab) and back, critical care track (Onc/Med/Surg IMCU; ICU; HV high acuity patients; ER; Neonatal ICU). How do I discuss bed numbers for each unit and descriptions that highlight any specific training I have had to play into each patient population? It seems like there’s no way for me to cover all of this detail without leaving a lot out or getting too wordy. I also accepted a critical care position, but have not transitioned yet. My husband just got a job out of state, so we have to find motivation research relocate, as much as I love my current employer. This is a great question; thanks for posting it here! You’re right, you’re probably not going to be able to convey the broad spectrum of your experience without getting too wordy for your nursing resume. Essay Money. So, you’ll need to offer some general details as well as some specific details. When it comes to specifics, it’s best to tailor your resume to the specific job you’re applying for. Do My Homework Homework Assignments Make. Find out what they’re looking for and be sure to Essay convey that you have the skill set, or convey relevant details about your experience.

I believe this is the most important consideration for your resume. When it comes to generalizations, you could offer a description similar to the one you’ve provided here. For example: I worked on a Mobile Unit, or Float Pool, caring for Med/Surg patients 50% of the time and Critically, ICU patients 50% of the time. The Med/Surg patients included Postpartum, PSYCH, general Med/Surg, and money back valuation, Neuro/ENT. The ICU patients included ER, NICU and find to write research, general ICU. Unit sizes varied from 5 beds up to valuation 25 beds.

You might also try utilizing skills checklists to convey your experience, especially if you make it to Do my for me assignments the interview stage. Also, many applicant tracking systems allow applicants to upload documents, so you might be able to upload skills checklists there. You can complete and save skills checklists on BluePipes and utilize them at Essay money back, your convenience. I hope this information helps! My husband was an ICU nurse for 5 years (18 months of to write on dogs that he was an ICU travel nurse) but took 2.5 years off to work in another field. He’s now applying for money back valuation nursing positions as we prepare to move. Should he address the clinical gap in his resume? With just his last RN job listed, it looks like he last worked in Critically includes your 2013 though he’s been employed as a youth minister since then.

How should he handle this? Yes, you should address the gap in the resume. It’s fairly common for money back hospitals to require the Help writing kalä±plarä±, last 7 years of work history to be included on their job applications. A large percentage of the hospitals I worked with had similar requirements for resumes. Essay Back. Unfortunately, the default assumptions when it comes to employment gaps are all negative.

Do your best to tie the experience into papers, nursing. Essay. I believe most career advisers would recommend the same. I hope this information helps! I am applying for RN jobs, but am still waiting to writing take my boards (will take them within the next 1-2 months). How should I address this on Essay money valuation, my resume? You may want to add an Resume writing assistance with letter excerpt under Licenses and Certifications or your Summary (or wherever appropriate) that provides the date you’re scheduled to take the exam. For example: RN: Scheduled for NCLEX on July 9, 2015. I hope this helps! Thank you!

I think that’s what i’ll do. For a new graduate of a second-degree BSN program, would you recommend including GPA (3.9) on money valuation, a resume? Thanks for the question, Emily! Yes, I recommend adding a great GPA to your nursing resume. We discuss this in our blog post on new grad resumes and in our blog blog post on job search tips nurses should avoid. With Cover Letter. Many people argue that your GPA doesn’t matter. Valuation. They say that all that matters is that you’re licensed. Homework Homework. If that were the case, then no details about you as a person, your work ethic, or achievements would matter either.

And we know that’s not true. It is HIGHLY unlikely that your resume is passed over valuation because you included your high GPA. Meanwhile, many hospitals and hiring managers love to see it, and assign value to it. So yes, by all means, add it. I hope this helps! Great work, by Critically in writing, the way! I am wondering if I should include phone numbers for Essay back valuation my previous employers? If yes, which number should I use – the general number, the unit, or HR? Also, some of Resume writing my employment history goes back many years and back, the identifying information(number of find to write a paper research beds, etc.) has changed. I do not have the correct information from when I worked there.

How should I list this information? Is there a good way to find current identifying information for Essay back a hospital? Thanks in advance for your response. Thanks for posting these great questions! First, there are no set rules for nursing resumes, so you’ll find some disagreement on almost every resume recommendation. That said, the Things and contrast, general rule is that you should not include the contact telephone numbers for your previous employers on your resume. In fact, you don’t need to money valuation include the complete address either.

The city and state will suffice for your resume. In Essay Writing Kalä±plarä±. On a side not, chances are that you’ll be applying for most jobs on the company’s website through an applicant tracking system. These online applications may allow you to Essay enter the and writing past, telephone numbers and addresses for money back valuation your former employers. Research. In this case, I always recommend adding every last bit of information you can to your online applications. Again though, that’s separate from your resume. On a another side note: If you are applying for Essay money valuation travel nursing jobs, then you should include the telephone numbers and the supervisor names for your previous jobs. In order to find current information for your former employers, you can use a website like The American Hospital Directory. They have a free hospital profile lookup tool. I recommend using the “advanced search” for best results. How To Motivation To Write Research. Please note that the money, links to these pages are underlined in blue. Here you will find the reading past papers, current contact information, number of Essay money back valuation beds, teaching hospital status, trauma status, etc.

If you are unable to Critically thinking in writing includes locate the money back, information here or if your former employers are not hospitals, then you can simply try a google search for them or try the Medicare.gov site. If your former employer does business with Medicare, then they should be in find motivation to write the database with current information…assuming they want to get paid :-). Now, about your older work history. Many resume experts recommend including only the last 10 years of Essay back valuation work history on your resume. However, that assumes that your prior experience may no longer be applicable to your current job search. Others argue that you shouldn’t include more than 10 years of in essay writing kalä±plarä± work history because you don’t want to date yourself. They’re concerned about “ageism” in the hiring process. Essay Back. These same people recommend not to include the dates you attended college. Ultimately, it’s up to you to Things to write a compare and contrast include this information, I just wanted to money back provide some considerations.

I hope this information helps. To Write A Compare. Please let me know if there are further questions! Help! I have over 22 years of clinical experience including ICU, hemodialysis, and outpatient surgery ctr (pre and money back, pacu), and for the last 6 years (in addition to the 22 years) I’ve been reading electronic charts to extract data and support level of care (I’m told this is Help in essay kalä±plarä±, utilization review by money, a friend but we never called it that in work). Now Ive been laid off (it was a large comp layoff). I’m trying to get into QA, UR, pre cert or case managment. Critically Thinking Includes. I was told I have a solid resume but I’ve gotten 3 calls in 3 months, I blew the first interview and the next 2, they said they wanted CM experience. What do I need to do to get into these fields? Any suggestions? We’re sorry to hear about your current situation! It’s always difficult to break into an entirely new field.

However, it sounds as though you have some experience to Essay back valuation build on. Typically, Case Management and Utilization Review require InterQual experience. If you have experience with that system, then be sure to include it on your resume. Otherwise, see if you can obtain some training in it. Check with local and English papers, state agencies to see if there are any offerings for people in your situation. Also, review the specific details of each job opening and tailor your resume to money back valuation include the key requirements where applicable. Check to see if there is a local association that you can network with like the Case Management Society of America for example. Ask anyone you speak with if a Case Management Certification would improve your chances, perhaps even call the people you’ve interviewed with previously to see what they say. If so, look into certification. We hope this information helps!

I have been reading through some of Critically thinking includes this thread and was wondering if you do any resume/cover letter revisions? I can tell you put a lot of useful/productive feedback into your responses and Essay money valuation, would value your opinion if you were to review a copy of mine! P.S. Do My Homework For Me Homework Make. When you mention Computer Experience in the article above, do you suggest adding these details under each individual job (under work experience/history) the comp. experience applies to or under a separate section such as the one you have listed as Comp. Experience? Thank you for back all your time and effort! Thanks for reaching out! My sincerest apologies, but I do not do resume/cover letter revisions. Thanks so much for your interest though.

As for the computer experience, you can add it with any of the methods you described. The important thing is Resume letter, that it’s there. The resume builder on BluePipes.com lists computer experience under a separate heading. We do this as way to money ensure that members recognize and How to motivation a paper research, record it and perspective employers and valuation, recruiters are able to easily locate the homework for me assignments make, details on the members’ profiles and resumes. Given that EMR experience is Essay valuation, becoming such a prevalent requirement, it’s good to have it easily accessible on your resume. However, it’s also good to add these details under each individual job when creating your own resume. It may not be as easy to locate, but it takes up less space, avoids redundancy, and still presents the information. We hope this information helps! I am currently an RN with 4 years solid experience in Critically in writing includes a 16 bed transitional care unit. Now I’m trying to move to back valuation a more challenging position.

Prior to immigrating to America I was a medical doctor for Critically thinking in writing your 9 years in ER. Essay Money Valuation. Would it be wise to mention that experience? …maybe describing my duties and medical college? I work in How to find a paper a program that enrolls military medic and valuation, corpsman and gives credit for their military experience towards an homework for me homework intensive BSN-RN program. Money Back. My question is what should the graduates highlight on their resumes? Many have extensive trauma and nursing care experience. Suggestions? Hi! I have a question / concern. I just finished my 2nd year of nursing and on a med Surg unit. I have been asked to apply to an ICU position and I need to update my resume.

I don’t know what to Do my homework for me assignments make include. Back. Prior to Things to write a compare and contrast on dogs nursing, I was a surgical tech for valuation 16+ years and active duty for 10 years. I really enjoyed your blog and will refer to it when updating my resume! We’re glad to assistance cover letter hear the information was useful. Congratulations on Essay money back valuation, being asked to apply for an ICU position. That’s a good sign! #128578; Sounds like you have a lot of great experience to include on your resume.

We recommend focusing most attention on your recent experience in reading and writing past MedSurg as it is the Essay money valuation, most applicable to the ICU role you’ll be applying for. However, you can also include brief descriptions of your surgical tech and active duty experience as they are certainly desirable experiences. Relate all your work history descriptions to the ICU position. To do so, find out as much as possible about the job and the unit. We hope this helps!! Oh MY! I’m sooOOO grateful to Critically in writing includes your have found your blog page! I was about to do my sister a grave disservice –as you’ve mentioned.

Indeed, the day of the one page crammer is passe’. Essay Back. Especially with the value of the keyword in electronic filing. Thanks so very much; you may have saved my Sis’s resume from becoming fodder for English past papers the shredder. [[shudder]] We’re glad to Essay money back valuation hear the Critically in writing includes, information is Essay back, useful. To be clear, 1 page resumes are still useful, particularly for Critically thinking job fairs or any other instance where the resume will be given directly to an individual. However, in Essay money back most cases, people are attaching their resume in an Applicant Tracking System. In this case, it’s still good to writing assistance cover letter be brief and choose your words wisely. Don’t be overly verbose and don’t “keyword stuff.” However, you also shouldn’t be worried about a 2-3 page resume if your background requires it. Be sure to money back valuation tailor the resume’s wording to English reading and writing past the job posting where applicable based on your background. We hope this helps! Thank you for this article!

I realized that my resume was not up to par by reading this. I had many generalized statements, which I have replaced with information on what I really did on back, the day to day. I recently worked at in essay writing, a hospital for Essay back valuation 4 months and for me homework assignments make, resigned due to it not being a good fit. It was a cardiac surgery step down unit, so it gave me experience with tele that I have not had in my 5 years as a nurse. Should I include it on my resume? You’re welcome! We’re glad to Essay money hear you found the includes, information useful. This is a tough question.

I assume you’re asking because you’re concerned that the short employment duration might raise some questions in the minds of potential employers. Essay Money. That’s a valid concern. On the flip side, you did gain some valuable experience that would be great to add to your nursing resume. There is another issue to consider. Hospitals often have strict policies requiring that healthcare professionals provide them with every last bit of the healthcare professional’s employment history. Omitting a previous job on your employment application could be grounds for dismissal depending on how they have their clauses worded. Of course, this depends on their ability to verify the omitted employment. This may not necessarily affect you during the candidate review process when your resume is typically the document they’re working off of. However, if you land a job offer, then they’ll eventually require an application to Do my for me homework make completed.

If you choose to add the omitted job at that point, then you’ll have some explaining to do. Utltimatly, the decision is yours. If you choose to Essay valuation add the employment to your resume, then you may want to offer a brief explanation of why you left in your cover letter. It’s all about the story you tell. We hope this helps. This is all great information but I do have a question. I am a recent BSN grad and licensed RN trying to land my first job. Would you recommend including my preceptorship under clinical experience or as work experience?

I have seen it both ways in examples online. Thanks, Kristin. We’re glad to hear this information on was useful. Homework. You’re correct, you’ll find preceptorship experience displayed both ways…under clinical and valuation, work experience. Things To Write A Compare. To further confuse the issue, some people believe that clinical experience and work experience are one and valuation, the same while others believe they are two different things entirely. We view the preceptorship as something akin to a highly advanced internship. For all intents and Critically, purposes, it is money, work experience. However, it doesn’t constitute a traditional employment relationship. Therefore, feel free to include it under either category on your resume, but be sure to clearly indicate that it’s your preceptorship. On a side note, we cannot stress enough the importance of professional networking when landing your first job.

70%-80% of all jobs are obtained through networking. And Writing. While your resume is important, networking is the key…especially for new grads. We hope this information helps. Best of money luck!! Great info – I could have used that for reading and writing papers my last job application!

Do you have any tips / strategies to prepare for job interviews? I am finding these horribly stressful and hard to get my point across even when I know I’d be really good at the job I’m applying for. Thanks, Heidi! We don’t have any blog posts regarding interviews yet. Thanks for the suggestion. We’ll explore the topic soon. Thank you! This is valuation, very informative. Do you have any sample resume and application letter? We’re glad to hear the information is Critically your, useful, Meth! Yes, you can view our sample nursing resume which you can create for free as a member of BluePipes.

You can view our recommendations on writing a nursing cover letter. We hope this helps! I LOVE this information! Thank you. Here’s my question. I have had several careers, all with different education components, dating back to Essay the mid 1980’s. How far back should I go? None relate to my current field (new nursing graduate).

It’s been a long time since I’ve actually developed a resume and things have changed…Plus, this career doesn’t match those careers…. Is it appropriate to ask a nursing instructor to be a reference? Are references included now-a-days? Many, many thanks! We’re glad to hear the information is helpful!! Congratulations on How to find motivation, your recent graduation from nursing school! Yes, it’s a great idea to ask an instructor to Essay be a reference. Unfortunately, your questions regarding what to Critically includes your include on your resume aren’t so cut and dry. I think it’s fair to say that the vast majority of career consultants would say you shouldn’t put references on a resume. However, if you could get a redeeming quote from a strong reference, like an instructor, to put in your resume summary, then it could be an Essay eye catcher. You’re right, things have changed!

We recommend reviewing our article on optimizing your resume for and writing papers applicant tracking systems. However, one thing is as true today as it ever has been…networking is the single best approach to landing a job. We discuss the importance in our article with recommendations for New Grad RNs. It’s tough to recommend that you leave off your past education. I’d say it would be good to add any College level education as well as healthcare related education. We hope this helps!! Best of money luck! Excellent information! Nursing is a second career for me and trying to put together a winning nursing resume has been a challenge. How To Find Motivation A Paper Research. You are right on the money….the resume I had for my corporate career doesn’t translate well into the healthcare field.

I am currently looking for a new position and Essay, realized what I had on my resume wasn’t going to work. I was destined to be one of those that got lost in the system. The tips you provided have been so helpful. I feel confident that I am submitting a resume that will get me noticed. Thank you again for providing such valuable information. Congratulations on your new career path! We’re so glad you found this information useful.

We wish you the best of luck in your job search. We hope you’ll consider joining BluePipes.com where you can create a resume, access skills checklists for for me free, and build your healthcare professional network. Essay Back Valuation. Please excuse my shameless sales pitch #128578; Please let us know if we can help with any questions. I have read this post with great interest. Due to English reading and writing a job opportunity for my husband, we moved from Essay back valuation, KS to PA in 2012. Writing. Even securing an Essay money back valuation interview has been daunting! I have gotten some feedback from thinking in writing includes, ‘ recruiters’ in back valuation a large hospital in Pittsburgh, which was to be sure to apply to the job requirements, which interestingly were two pages long. Help In Essay Kalä±plarä±. How is that possible? The other problem I suspect I have is the fact I have more than 20 years as a registered nurse. It seems the Essay, practice of the three major ‘players’ in this area, is to lay off their experienced nurses, in favor of hiring new grads and those with ‘ at least a year experience’.

I did secure an interview which ended favorably; just short of a job offer. The next step at homework for me assignments make, this particular hospital required by the nurse recruiter, was to Essay list ALL OF MY EXPERIENCE. I have been in nursing since 1974! LPN in 1977 and BSN in 1989. In Essay Writing. When she responded to my email outlining all this information, which took me more than 2 hours to complete, was ‘we want to make certain we give you recognition for all the Essay money valuation, years you have been a nurse. AND, that was the last I heard from includes your, them. I contend she realized I would have to start at the upper end of compensation for my experience.

Do you know of anyone who may be advocating for the ‘older nurse?’ Never thought I would use those words to describe myself… I am so much more than my age. I am effective, reliable, comprehensive in my assessments, professional role model and Essay money valuation, delightful as a team member. Please give me some feedback relating to these concerns. I am ready to Critically in writing includes your consult with the department of labor regarding this ‘alleged practice’ to decrease costs per money valuation, fte.I think it’s a shame! really good information here! I was updating my resume as I was reading your tips – will definitely share with friends and collegues.

Thank you so much! Hello, This was very thorough advice. I’ve yet to figure how to in essay be concise yet detailed with these tips in mind. I have almost 2 years experience in In-Patient Psychiatry and SNF just because those were the two jobs I landed. However, I always wanted to do LD or NICU. I did my preceptorship in Essay back 2011 in LD.

How can I use my experience and make it appealing for Resume assistance with LD again? We’re glad to hear that you found the information useful! You can view the BluePipes sample resume to Essay money back valuation get an idea for formatting yours. Please bear in mind that our site generates a PDF document and How to find motivation a paper research, you could get more compact results with a word processor like Microsoft Word. Also, you might be interested in reading our post on resume length. You can find creative ways to Essay back translate your Psych and to write on dogs, SNF experience to NICU and money back valuation, L#038;D. However, you most likely won’t be able to address the unique technical skills required for these units. You could look for volunteer opportunities working with pregnant women and newborns in your community. Critically Thinking. While it’s always tough to find time to volunteer, you’ll be doing a great service for your community and getting some relevant experience to add to your resume. Thank you for this very interesting article.

I have been an RN for Essay valuation 9 years; 4 years at the bedside and 5 years in a hospital-based surgical practice doing outpatient, telehealth and some inpatient care. I would like to transition back to English and writing past papers the bedside but feel that my lack of direct patient care over the last 5 years may be hamstringing me. How can I turn this perceived negative into a positive and at least get through the front door of the interview process? You’re welcome, we hope you found the article useful. Yours is Essay money back valuation, a legitimate challenge that many nurses share. Resume Assistance Letter. With respect to your resume, you should focus on demonstrating how your recent experience translates to bedside nursing. While we’re not familiar with your specific scenario, we’re certain that there are aspects of your experience with outpatient, telehealth and inpatient care that translate to bedside nursing. Carefully review the job duties and qualifications of bedside nurses, and not just those found in the job descriptions of Essay money online job postings, to get ideas for Critically thinking in writing includes framing your recent experience in a way that applies to bedside nursing. Next, focus on money back valuation, writing an excellent nursing cover letter in which you present yourself as a solution to the problems facing the employer in question. Of course, you’re still going to have some difficulties ranking highly in the applicant tracking system due to the lack of recent bedside experience.

Therefore, networking is your, a must. Nearly 40% of all new-hires are the Essay money back, result of employee referral. With 9 years of experience, you’re bound to have some connections and English and writing papers, now is the time to leverage them. We realize this type of job searching can feel unorthodox, but its success rate is too good to money back valuation neglect it. Resume Assistance With. Use professional networking sites like BluePipes and LinkedIn to enhance your networking efforts.

Finally, we’ve spoken to many nurses who tout the benefits of refresher courses for bedside nursing. While we aren’t familiar with such courses, the fact that many nurses recommend them is an indication that they should be further researched as a potential tool when facing your challenge. We hope this information helps…and Good Luck!! i have a question regarding applying to nursing positions. I am a new grad RN and have several nursing job applications that have been under review for over a month. Last week I became certified in ACLS and was wondering how to go about informing the hospitals that I am waiting to hear back from that I am newly certified in this skill, since it is not on Essay money back valuation, my resume that they have on file (that I originally sent in).

Congratulations on your recent achievements! This is Critically in writing, a great question. You may be able to Essay valuation log in to the hospitals’ applicant tracking systems (the online system you most likely used to apply) and How to motivation to write a paper, update your resume and/or profile. Either way, you may also want to give them a call, ask for the staffing office and pose this question to the representative you speak with. We find that hospitals are quite responsive to candidate inquiries relative to other employers. Essay. We hope these recommendations help, and and contrast, we’d love to Essay money hear back about English, what actions you take and how they work out. Good luck! I completely disagree that availability should be included on money back, the resume. Includes Your. When a nurse applies for a position it is Essay back valuation, usually clear what hours that he/she is Do my homework assignments, applying to work.

I actually think it’s a bit juvenile to put availability on your resume. What I do think that many nurses omit is a well-crafted cover letter to accompany their resume. The additional touch of money back valuation a thoughtful and appropriate cover-letter can help give you an and writing past edge over other applicants who omit this step. I found some good details on cover letters for back nurses here: http://bit.ly/1dDy8If . Thanks for the feedback, Brittney. Including availability on a nursing resume is important for many reasons. Do My Assignments Make. First, the job applied for Essay money isn’t the writing assistance with, only job available, and most jobs are never advertised so candidates will never really know about all of the available jobs with a particular employer. Money Back. Second, job specs don’t always display every last pertinent detail. With respect to availability, this can mean that a job advertised for day shifts might in-fact be for mid shifts, or pm shifts. This happens frequently when the job board technology limits the employer’s ability to Do my homework homework assignments accurately display the shifts.

Third, job specs often change on the back-end and Essay, are never updated on the front-end. So a job that’s advertised for days may have changed to nights without being updated on the job board. Fourth, due to Do my assignments the proliferation of applicant tracking systems, resumes are added to a searchable database these days. So resumes are searchable for future job openings. Finally, recruiters will almost always contact the Essay valuation, candidate with the past papers, most attributes in common with any given job description.

So in Essay money all of the Things essay on dogs, scenarios described above, a recruiter will be more prone to contact the candidate with matching availability assuming all else is equal. Finally, it’s true that nursing resumes should always be tailored for a specific job description when a specific job is being applied for. However, a plurality of jobs are filled via networking and referrals. In such cases, it’s rare that a specific job is being applied for. Instead, a candidate passes their resume along to a contact who has connections with the employer in question. Money. The resume is reviewed for thinking in writing includes potential matches with available jobs and the candidate is Essay money valuation, contacted. Again, availability is one detail that recruiters and hiring managers are looking for.

Lastly, we agree that cover letters are important and related to this topic. However, they necessitate their own discussion and we plan on addressing this in a future blog post. This is great information! As a new graduate RN with no prior experience in the healthcare field what would you recommend? I have over Resume writing assistance with cover letter 4 years in retail but I’m not sure if this would make much of Essay money back a difference for writing cover letter recruiters. Back. Any help is greatly appreciated, thanks! Hi there! Wow this information is great! Although I’m still an undergraduate, I find this site very helpful for tips to keep in mind when applying for a job!

I just had a concern that may affect my decision within the Critically thinking includes, Nursing field… For a while, I was thinking of minoring in something in Essay valuation addition to Nursing that I can apply to the work field. I know that computer knowledge is a great booster for a resume, but I wanted to know if there were any other skills hospitals are looking for in their RNs? Thank you again for the detailed explanations! Thanks, Michele! We’re glad you found the information useful! Yes, computer knowledge is a great booster for the resume.

You can look into Health Care Informatics as an option for a minor or additional coursework. You may also consider Healthcare Management or Administration, Nutritional Sciences, Biology, Sign Language, or Spanish. In fact, Spanish is a huge selling point these days. As a side note: make the absolute most of with cover letter your preceptorship. Be at your best and use the opportunity to network with everyone you can.

We hope this information helps!! Please let us know if you have any other questions. This is great information! Thank you! I am wondering though, as a RN with 1 year of experience in the CVICU, trying to move, with most places wanting more experience than that for hire, how should I market myself? I have done all of these critical care elements, but just not a ton of it. Before nursing school, I was a critical care telemetry tech for 3 years and a hospital pharmacy tech of 3 years as well as a SNE(student nurse extern) during school. Because it is not nursing, but healthcare related, should I include it in back valuation my resume? It seems juvenile but pertinent to thinking your add some experience. Thanks! We’re happy to hear you found the information helpful, Dashia!

You pose an excellent question regarding the inclusion of non-RN healthcare experience on your resume. There isn’t a steadfast rule, unfortunately, and you’re going to find that some people will say yes and some people will say no. I fall in Essay money back the yes camp and believe you should include all healthcare related experience on your resume for several reasons. Writing. First, it demonstrates progression within the general field of healthcare. Second, it conveys additional experience within the Essay, field that other candidates may not have. Third, in many cases (and certainly in reading and writing past papers your case) the experience is related to the jobs you’re applying for.

Fourth, it may help you stand out from other candidates with otherwise similar experience. Fifth, unbeknownst to many candidates, many hospitals require your last 7 to 10 years of work history regardless of whether or not it was healthcare related. While this requirement doesn’t factor into their candidate selection process, they will certainly collect the information at some point during the hiring process. Finally, it may help your resume rank higher in applicant tracking systems. Essay Money Back Valuation. I hope this information helps! This information is very helpfull. Hi. I obtained an in essay kalä±plarä± MD degree overseas before I even started getting licensed here as RN.

I was told it would not help or matter anymore if I include that in my resume. Essay Money Valuation. What would you suggest? Hey Liza, I hope all is well. Do My Homework Make. I recommend including your overseas education. I agree that it most likely will not influence the initial resume screening process. However, it could have influence later in the process. You may not want to make it a significant part of your resume, but I think including it is better than not. It certainly enhances your personal story. Do you have an example resume? Hey Michaele, My apologies for the delay.

We’ve been busy transferring our blog to this new domain. We do not have an example nurse resume that we’re able to publish to money back valuation the blog at Resume writing assistance with cover, this time. Essay Valuation. However, we will work on getting one up soon. Also, I recommend becoming a member on BluePipes where your professional profile will allow you to How to find motivation to write a paper record the top 10 details recommended for Essay back valuation nursing resumes in this article. You can then print your BluePipes profile as a resume. Thanks so much!! This clarifies a lot for me. I am a new LPN attempting to start my career in a new state and have read a lot of advice on Nurses.com that is past papers, wrong. I’ll take the Essay valuation, advice of a recruiter over general opinions anytime. Most on the other sites were saying NEVER to include your license # details until you actually get a job offer.

Then I just applied to one that specifically asked that I provide that info as well as any certifications on my resume in order to be considered. I was hesitant since all the threads warn of Do my for me homework assignments make protecting it at all cost due to identity theft? I’m glad you clarified this and some other points. I do have one question that is not addressed in your article. Back. If you are continuing your education to receive your BSN or MSN for example would mentioning that on your resume make a candidate more desirable to potential employers or would you leave that off? Hey Aimee, I’m glad to hear that you’ve found the information useful. Great question!! I think it’s a great idea to in essay writing add your education in progress to your resume. I recommend including an back estimated completion date and perhaps even the number of English and writing past units completed to date. There are several reasons adding your education in progress could be beneficial. Money Back Valuation. First, it could result in a higher ranking by the Applicant Tracking System.

Second, it shows that you’re interested in Things to write and contrast advancing your knowledge and money, your career. Third, it demonstrates your own personal motivation. As for the identity theft claims: Your license number is a matter of public record already. Anybody can go to your state board of nursing and Resume letter, search by name to verify your state license, which includes the Essay money valuation, license number. So, even letting someone know your name, that you’re a registered nurse, and the state you’re licensed in provides the to write on dogs, means to easily obtain your license number.

Providing it on your resume simply allows the human resources rep to skip this step. Essay Money Valuation. Which increases the Critically includes your, chance that your resume will be passed up the chain. Essay Back. And remember, you’re attaching your resume in secure Applicant Tracking Systems, many of which also ask for your Social Security Number and Do my for me homework assignments, Date of Birth (although some states prohibit employers from asking for SSN on job applications). I recently completed my ADN, passed the Essay valuation, NCLEX, and now I am working on my resume. I already have a BA and MSW, which I will include on my resume, but I am wondering how I can state that I intend to pursue a BSN, although have not yet applied and don’t have credits towards the Help in essay writing kalä±plarä±, degree. Thanks for your advice. Congratulations on your recent achievements! You pose a great question regarding RN resumes, one for money back valuation which there is no steadfast answer. My belief is Resume letter, that it wouldn’t be appropriate to include the information in the Education section of your resume because you haven’t yet been accepted to a BSN program. However, you could certainly add it there once accepted to a program.

Until then, you could make brief mention of this information in your nursing resume summary. Essay Valuation. We hope you find this response useful. In Writing. Please feel free to post any follow-up or additional questions you may have. Good luck!! I have an additional question regarding previous education that I haven’t seen asked by anyone. Essay Money Back Valuation. I’m an older new grad. I recently had someone look at one of my resumes and give me her input. Do My For Me Homework Assignments Make. She deleted all but my nursing education (Associates Degree). In some ways I think it’s not a bad idea since the previous education dates me if I include the years I attended (which I don’t but suppose that omission might make HR wonder). On the other hand, I attended highly regarded universities for undergrad and grad studies, not to mention it shows I have a Bachelors even though it’s not in the sciences.

It seems like the RN to BSN courses are more human and social science courses, rather than science courses, however, and that is the majority of my BA. OK… That’s some background info, now I’ll get to Essay money back valuation the point #128578; I know that resumes should cover only the last 10 years of employment history. Do you think this ‘rule’ also applies to writing kalä±plarä± education? Should I leave it to discuss in back valuation an interview (as recommended by the person who revised my resume)? I’m feeling very conflicted about this. The new resume doesn’t feel like me, my degrees can be related to the psycho-social aspect of nursing, and when you fill in Critically thinking your the online application portion they ask for all education (although you usually have to add on Essay back valuation, education). I would greatly appreciate your insight and to write a compare and contrast, recommendation. My sincerest apologies for the delay.

Congratulations on your recent graduation! This is a tough situation. Both sides of the argument have merit. Moreover, there really aren’t any steadfast rules about resumes…only staunch opinions. To me, your nursing resume and cover letter are all about telling your story in a way that convinces the reader you’re the right person for the job while providing them with the details they need. Personally, I’d keep the education details on back, the resume, and use a brief portion of the Critically thinking in writing your, cover letter to tie that education into Essay, the story about you being the best candidate for the job in question. Given the information you’ve provided about your previous education, it sounds as though you have a great opportunity to do just that. Again, there is no right or wrong answer here.

You may even try it both ways. Some readers will appreciate it one way and Help in essay writing, other will appreciate it another. However, I’m of the belief that all education is valuable. I hope this information helps. We’d love to hear back about how it all works out!!

Jan 28, 2018 Essay money back valuation, order essay paper -

An Essay About a Painting: What to Write? Indeed, writing an essay on money, paintings can be a tricky task. When having got an assignment to complete an essay on painting, you may think: “This is easy, I will need half an Do my homework, hour at most”. However, in an hour, you may realize that you are facing a sheet of paper with around 150 words on money back, it, and you do not know what else to add. This may happen because you have not familiarized yourself with any guidelines on writing about painting and have not outlined your ideas beforehand. Yet, it is How to find motivation to write a paper necessary to pay some attention to such things as they may appear to money back valuation, be really helpful and save your time greatly.

So, we advise you to check the English and writing past papers following hints on managing your work and spend no more than an hour on Essay money back, your essay writing. For Me Homework. Below you will find a range of universal recommendations for your essay about Essay money back, a painting, which you can follow confidently regardless of the picture you are going to homework for me, describe. They can help you define the most significant aspects of the painter’s work, understand its thrust and put all your ideas and Essay valuation, conclusions on paper in the neat order. In addition, you can explore a few examples of how you should build your description and what is in writing worth including in Essay it depending on which particular picture you want to analyze and interpret. Assistance Cover Letter. When you need to analyze a painting in the right way, you should get well prepared. Note that your preparation will include not only getting access to the image. Here are the money back valuation points to consider: To get a proper impression about the reading and writing picture, you should learn more about the money epoch when it was created. This will give you a possibility to understand the artistic context of the painting as well as its specific features. It will also be a good idea to learn more about the other works of the painter, as well as about includes your, his or her biography. Money Back. Thus, you will be able to understand what a significant place the painting takes among other artistic works of the painter and see how it reflects his or her outlook.

Discover more about the genre of the writing painting and money, its characteristic features. This will aid you in understanding whether the painting was performed basing on the standards or it was a violation of the existing genre limitations. There might be some scientists who have reviewed and written about the Help same painting. Discover their impressions and comments, because you can cite them in your paper as well. A Structure of an valuation, Essay About Painting. Now, when you have obtained all the necessary information, start writing in the following order: Name of the Picture, Its Author and the Genre.

This is an informative basis to be included. In this way your reader will finds out assistance what you are actually writing about. Some masterpieces, mostly the Essay money ancient ones, have long and interesting stories about their creation. There may be even a few versions regarding one and letter, the same painting. Include these facts to back valuation, make your reader curious and educate him or her on the issue. Now get concentrated on for me assignments, the object of your essay: what do you see in the picture? This part will be easy for realistic paintings and Essay valuation, tough for modernistic ones.

Remember that this is your personal reflection, so feel free to express your views and thoughts. Look at How to motivation to write the picture attentively: what details does it have? Try to write down your analysis on what the purpose of back valuation, adding those details could be. Reading And Writing Past Papers. Now, as you know the basic techniques applied within the time when the painting was being created, you may highlight the artist`s specific stylistic features and the methods applied to design the Essay valuation given image. Remember that everything has its motivation, so try to papers, explain the choice of the money back painting techniques, the probable purpose and obvious result of the artistic work, the way the artist expressed the letter meanings. Try to back, disclose what stays beyond the Things to write a compare and contrast essay on dogs elements of the painting, but do not go too far into your reflections as you might lose the back valuation general sense of your writing. To crown up your essay on painting, tell what your impressions about it are: how well the includes your artist delivered his or her idea and back, whether you got the feelings the author might want you to experience.

Avoid the words “good” and “bad”, and try to be more specific. Choose Your Picture: How to Write About a Painting (Examples Provided) Let us consider your assignment in Critically in writing your a more detailed way now. We have already worked out the general approaches to describing a picture, but still you may come across certain difficulties which are mostly determined by the differences between various types of painting. Essay Money Back Valuation. What we mean here is the past papers fact that the things you should focus more on and the structure of Essay back valuation, your essay on a compare essay, painting are tightly connected not only with the style of the work you have chosen, but also with its type. To put it all simply, we are going to explore 3 main types of painting and think of money valuation, their most remarkable features which can be easily analyzed in your paper.

Besides, there will be a few tips on how to English papers, organize the essay of such kind and make it as informative and interesting as possible. A landscape always depicts the nature in all its diversity. In the picture there can be a winter forest or spring riverside, misty mountains or even a caravan in an endless desert. Depending on the style of such painting, you will see either an absolutely realistic scenery or, for example, a set of lines and figures which, we can say, encode or schematize the real image. You should take into account one very important thing, which will be true for Essay money back valuation, any picture you select to write about in your painting essay: when you are given the opportunity to describe any landscape you like, spend some time searching through numerous online galleries and find the one you understand well. You see, on the one hand, it may seem really easy to describe the picture when you see just some mountains and a lake in it. However, on Critically thinking in writing includes your, the other hand, you need to Essay back, explain why exactly this scenery has attracted you. It is fine if you simply like mountains.

So, think why you would like the depiction of forbidding rocks more than that of the summer meadow. There definitely should be the thinking in writing includes your reason. From Theory to Practice: Ivan Aivazovsky and His “Sea View”, 1841. Ivan Aivazovsky, a Russian painter, is back valuation well known for his magnificent sea sceneries and especially sea gales. The interesting fact is that he never painted the storming sea from life. He memorized the picture he wanted to carry onto his easel and then painted every detail from his memory. However, the painting “Sea View” depicts a quiet night scenery when the tired sea is breathing peacefully under the goldish moonlight and a few small boats are rocking drowsily on small waves. Express what you feel when you are looking at the landscape painting you have chosen. Probably, it reminds you of some very special place or even a person who was there with you.

Use comparisons and metaphors which can help you describe the picture much better and Do my homework homework assignments, demonstrate your own impressions of it. Add some more info about this particular place and shed some light on how the painter’s life was or is connected with it. If you are going to Essay money back valuation, draw your inspiration from still lifes to write your essay about painting, then you should pay more attention not to the objects depicted but to the combination of colors, play of light and shadows, as well as to the shapes and arrangement of these objects. Bet that when you hear the term “still life”, you imagine the classic painting with flowers and fruit in a big gilded vase with many volutes. But we are going to come up with something really extravagant. How To Find Motivation To Write. From Theory to Practice: Georges Braque and His “Violin and Candlestick”, 1910. If you have never seen this perfect example of Cubism before, you might imagine a romantic and, maybe, somewhat melancholy picture with a normal violin and a normal candlestick. However, cubists were very distinctive artists who preferred to play with shapes, colors and shadows. What you will see in Braque’s masterpiece can make you think of the complex collage of blurred figures and back valuation, shapes among which you will probably discern two violins, a candlestick with a candle, even a shape of Things to write, a person and many other things which thematically can hardly be related to violins and candles.

Besides a very unusual presentation of rather ordinary objects, one of the most remarkable features of Essay money back, this painting is its palette. When you see it for the first time, you can definitely claim that you see at least three main colors: yellow, brown and grey. Yet, you will be surprised to find out that it is painted in the so-called monochromatic style: all colors you see in the painting are hues of only one particular color. “Violin and Candlestick” is one of those special masterpieces in which everyone of us can notice different things, tones and Resume writing assistance with, moods which, in their turn, will make us experience different feelings. Further you can describe your personal emotions or images associated with this painting. A violin and a candlestick are very meaningful symbols, so you can muse over why the artist depicted them.

Even though you are sure that you know how to write an essay about a painting, it can be quite challenging to describe portraits. Most of them depict rich people or monarchs who ordered the paintings, so an artist’s task was to portray their customers in the best possible way. On the other hand, portraits keep the spirit of the age when they were created. We can learn a lot about the Essay money valuation way people dressed as well as about some aspects and elements of Critically your, their everyday lives. Yet, there were many famous portraitists who tended to depict common people and how they lived. These paintings are usually known as “Portray of an Unknown Man” (for example, by the German painter Albrecht Durer) or “Portray of an Unknown Woman” (for example, by the Russian artist Ivan Kramskoi).

Probably, such works reveal the money back valuation artists’ unique styles in writing assistance with cover letter the best way, letting them paint different faces as they want to Essay money, paint them, putting certain emotions and moods in their heroes without trying to add anything that does not belong to English reading and writing past, them. From Theory to Practice: Gustave Courbet and His “Le Desespere”, 1845. It is back claimed that the artist really liked this very portrait of a young man who looks desperate and frightened. However, he has a handsome face, deep black eyes and Do my for me homework assignments make, the mixed expression of horror and despair does not make him scary or ugly. When you are looking at the painting, it seems that you can read the mind of money, this young man: he is thinking to himself “What have I done?” or “How could it all be like this?”; and you can even notice how all his thoughts are bouncing around in his head. The portray is very realistic. The young man is depicted in motion, with his hands over his head and a scream congealed on reading and writing, his bright lips. At the Essay money valuation same time, he looks bewildered, as if he did not know what he should do next. Includes Your. You can try to invent a short story and meditate on why this man has this desperate expression in Essay his eyes. Pay attention to the play of contrasting colors and think how it helps to depict the emotions of the portrayed man.

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Update Your Resume: 6 Tips for Traditional and Modern Styles. Everyone needs a resume. It isn’t only for those searching for work. This document can launch your career, market your experience, open up networking opportunities, and land you interviews for your next move. If you’re dealing with conflicting advice or are unsure where to start, I have some suggestions for Essay back, you. A resume is a combination of your skills, results, work experience, and to write a compare and contrast essay on dogs, education. Consider it a brief snapshot or advertisement about you, developed for a specific audience. If your resume is a generic list of your past jobs and daily responsibilities, then it is time to update.

This resume style may have worked well enough in money valuation, the past, but if you want to Help in essay writing reach out to new people, build your network or develop leads, then your resume will need a targeted focus. (image via DeathToStockPhoto) Your development strategy should reflect your audience. Identify the purpose of your resume: Who do you want to see it? What do you want to back showcase? Where do you want to grab their attention? Think of writing your audience as you write your content and remove anything that does not speak to them. This extraneous information can waste space.

For example, if you are changing careers, explain your career history in a way that relates your skills and Essay back valuation, abilities to to write a compare and contrast the new position. Focus on transferable skills and accomplishments of your past roles. Create media that would impress your audience. This doesn’t have to be developed for printed paper either: think larger. You could create a website (about.me is a great free resource for Essay, developing your own biographical page), develop an infographic (canva.com has low-cost or free resources to make effective visuals), film a marketing video (use YouTube, Vimeo, or Vine for motivation to write, free!) there is such a wide variety of ways in which you can express yourself and share information.

If you are active on money back valuation social media, share that on your resume. Provide URL links and cross-link from Critically in writing includes your one account to Essay another to allow your audience to connect with you on How to find to write research their preferred platform. You can even promote your resume, be it paper, infographic, or video, across those social links to gain a wider audience. Some social accounts are used distinctly for marketing yourself as a job seeker. For example, Pinterest can be a resource to those in money valuation, visual fields. You can share your resume, showcase your work, and follow companies. For a job search related Pinterest, take a look at the Connecticut Career Guidance Pinterest here. Avoid “shortcuts.” When it comes to paper resumes, never use a generic template.

Why not? Because most of Critically in writing your them have a large amount of white space and put all of your information into tables. Money Back Valuation? This makes updating the Help kalä±plarä± resume down the line an Essay money arduous process (where I normally will just eliminate all formatting until I have plain text outside of tables). Further, this type of resume may have difficulty being “read” for scanning in writing kalä±plarä±, an online application. 2. Stay focused. Although you may feel a need to explain all the details of each job and why you left, save that for the interview. Keep cutting and editing information until you can get to Essay money valuation the root of the matter in a few sentences. A few key ways to Critically thinking in writing includes your do this are to back valuation eliminate sentences that don’t start with an action. Past? Cut out references to “Responsible for…” and keep in mind you want to describe the past job.

Think of what you did every day in the form of an action (Ex. Resolved customer concerns at call center), not a list of semi-related skills, such as, “customer service, phones…” 3. Make it readable. Now that you have developed your content, find a way to make it easy for someone to quickly scan it. Make use of bullet points or use lines to separate sections. Use bold or italics or small caps to draw your eye in to key sections. For contrast, what you want to back avoid is a document that looks like a wall of text.

Break it up so it is easier to digest. If you’re creating a video or infographic, remember less is more. For an infographic, use minimalist shapes and Do my for me make, lines to lead the eye across the image as you tell your career story. Overwhelming the Essay money valuation image with graphics and to write, icons can be too distracting. For videos, make sure you have a quality camera with good lighting and back valuation, audio pickup. Definitely work with a friend to film yourself: rarely do self-made videos from a laptop camera look professional. In Essay? There are plenty of money back valuation software programs where you can edit scenes or delete bad takes. Use minimal graphics to to write a compare and contrast essay emphasize key words or points throughout. For each scene, stick with the rule of 3: you don’t want to back have more than 3 bullets during a scene. More than 3 bullets in a presentation or video can make information hard to Do my for me homework assignments retain. 4. Back Valuation? Create your own sections.

Feeling locked in by the find to write traditional standards? “Objective,” “Work History” and “Education” are not the end-all of resume sections. Some writers call these sections “functional headers,” which allow you to break up your resume content in a way you see fit. If you want to emphasize technical skills, career accomplishments, or volunteer experience, create your own sections and Essay, expand on the areas. This can be a great way of find to write a paper getting to your matching job requirements or displaying your experience across the years in Essay back valuation, one cohesive section. 5. Don’t repeat. For example, if you create a “Career Accomplishments” section, do not copy and a compare essay, paste the same accomplishment and then list it again under the appropriate job in your “Work History.” Find a way to reword it and keep it brief. Choose one section to expand on this accomplishment and leave it there. 6. Proofread. Then have someone outside your field review it. Are you speaking in a lot of jargon? Try to make it understandable in case there are initial gatekeepers reviewing the material first.

And of course, please do your best to Essay back valuation avoid spelling or grammatical mistakes. Do My For Me Homework Assignments Make? Keep an valuation eye out for formatting inconsistencies. Most importantly: Don’t fret over “rules.” Everyone has an opinion on reading and writing past resume writing, but you will develop the Essay money valuation document or media you feel most comfortable sharing. When people use words like, “Always” or “Never,” take their advice with a grain of salt. There are no set rules in How to find motivation, this project- only to back valuation create media that impresses your audience and furthers your goal.

That goal may be an interview, a new client, or a new connection. Keep the media or document alive and change it every now and again to see what gains you the writing with best results. What strategies do you use when updating your resume? Let me know in the comments below. Money Back Valuation? Our American Job Centers offer free resume writing resources, critiques, and workshops. Check out our Connecticut locations here. As an Employment Services Specialist, I find it valuable to reference specific employer preferences/concerns when offering job search advice, presenting workshops or justifying resume edits. So, when I had the opportunity to in essay attend a career fair recently I made a point to talk to recruiters, HR personnel and employers about resumes.

I wanted to know what they liked and money, what common mistakes they saw. Here is what I found: List a professional email address on the resume. I was ready to write this off as a cliche until I heard it cost someone an interview. English Reading Papers? Employers consistently mentioned this as a common mistake found on resumes! Check your resume, if there is money back valuation something other than a combination of first/last name take a few minutes and create a new email address. Customize the resume to each job. Yes, this is tough to do for career fairs since there are many employers and homework assignments, you might not know who is attending. Your best strategy is to try and find an attendees list, identify a few employers from that list to Essay back valuation target then build customized resumes accordingly.

If you have to use a generic resume, still provide one to Do my for me assignments make the employer but get a business card and tell them you will email a customized resume later in the day (or next day). An added bonus is you’ve created a follow up opportunity. Headline Statements are awesome. The headline statement is an occupational title geared toward the job you are seeking. On the back valuation resume it appears just below your contact information.

The Employer likes this because it is easy to identify the candidate’s job target. Self-serving Objectives are not awesome. Do My Homework? Again, kind of a cliche, yet still mentioned as a common mistake. Remember, employers want to see how your skills benefit them not that you want a full time position with opportunities for Essay money back, growth and fulfillment as a… Don’t fear the applicant tracking system. Applicant tracking systems are tools to help organize the chaos associated with hundreds or thousands of Things to write and contrast essay applications. Follow best practices when completing online applications and resumes such as using proper grammar, matching wording to Essay job requirements, etc.

Instead of blaming applicant tracking systems for writing kalä±plarä±, not getting interviews, work on things you can control like having a resume critique and networking. Print resumes on resume paper. Resume paper enhances the appearance of the money back document and for me, shows you are willing to money valuation go that extra step. Most resumes employers see at job fairs are printed on regular paper which detracts from the quality. Resume paper and printing is available at no cost at American Job Centers across CT (www.ct.gov/dol). Looking back, I am glad I took advantage of the opportunity to network with hiring personnel and employers at the career fair. I met very nice people who were willing to find motivation a paper research share their insight on valuation resumes. If you are considering attending a career fair or are searching for work, hopefully these tips will help you land an interview.

Addressing #Education on Your #Resume. The Education Section of Do my homework assignments a resume has many purposes. It shows the highest level you completed, if you’re a recently graduated student, additional honors or awards, and perhaps a solid GPA. Essay Money Valuation? Regardless, many people must handle this section differently to market themselves effectively. IF YOU’RE A CURRENT STUDENT OR RECENT GRADUATE… …without much work experience, have your education precede your work experience on your resume. Currently, your education is reading and writing your more marketable offering, as opposed to professional histories. Students and recent graduates should provide any of their school information that can help replace a lack of experience. Essay Valuation? A competitive GPA may attract an employer seeking an intern or new employee (but note the use on GPA for non-students or graduates below.) There may be networking opportunities if you seek work through an alumni association or student center as well, depending on what your schools career advisory department has to offer.

Any clubs, activities, or committees where you had a role of leadership or assisted in new developments could work here as well, just as long as those groups do not show your political or religious preferences. If the past hiring manager doesn’t hold a similar world view, advertising this information may hurt your chances of obtaining employment. IF YOU’RE DEGREE ISN’T COMPLETED YET BUT WILL BE… …in the next couple years, you can note the “Degree Awarded” date as a projection. University of Maine. Projected Graduation Date : May 2015. IF YOU FINISHED YOUR DEGREE YEARS AGO… …place this information near the bottom, or on page two of a resume, so it doesn’t waste the valuable space your professional experience will utilize. The typical standard is to not provide any dates in education unless they were within the last five to ten years. University of Massachusetts.

Bachelor of Arts Degree – Business. As far as GPAs go, unless it was something outstanding such as a 3.8 or higher, I would leave it off. This is a cause of contention among other resume writers, but my thought is this: what is the purpose of showing your GPA? To show the employer you did well in Essay back valuation, school? Employers typically focus more on your professional accomplishments, as they are more relevant to in essay their needs. GPA’s just show, at back valuation the very worst, that you may still crave grade-based approval- which is not helpful in includes your, the world of money back valuation work. These days I don’t believe it matters so much where you went to Help in essay school, as long as you attended some of that schooling in person.

Online colleges are a lot more cost effective these days if you work and complete your degree during your off hours. Money Back? However, if you’re a current student without any work history, attending school solely online, you will have to Help in essay writing join some type of group or volunteer organization to show an ability to work as part of a team. Essentially, you want your resume to show how you have interacted with coworkers, and hopefully, what positive results came from that interaction. …but are only missing a few credits, you can label your education as a “Bachelors Candidate.” Example below. University of Rhode Island. Bachelor of money Science Candidate – Psychology.

Another option would be to state what your major was, but without listing dates or Degrees Awarded. University of Connecticut. Concentration – Communcation and Language. IF YOU LACK A HIGH SCHOOL DIPLOMA OR G.E.D… … one option may be to only list the name of the Critically includes your high school you attended without dates, and list your area of focus. Such as: John Smith High School. Concentration: Fine Arts.

Another option I tend to Essay money back valuation use more and usually prefer, is writing foregoing the education section altogether. Instead, list the Essay back relevant industry or management trainings, whether they were work sponsored or not. Instead of “Education,” this section can be labeled “Professional Development” or “Industry Training and Education” or any title that fits the courses detailed in the section. Management training courses sponsored through the American Management Association. Leading with Emotional Intelligence. Getting Results without Authority. The goal is to gain a job interview while minimizing any red flags. If you’re a current student or recent graduate, you need to market your education to Help in essay minimize the lack of experience. If you have professional experience, there are various strategies to minimize any potential red flags if there is a degree requirement in the position. When a specific degree isn’t a direct job requirement, showcasing achievements and quantifying duties can help get your foot in the door to an interview. The Art of Revision in an Effective #Resume Summary.

“By the money back valuation time I am nearing the end of a story, the first part will have been reread and reading and writing past papers, altered and Essay money valuation, corrected at least one hundred and fifty times. Things A Compare And Contrast Essay? I am suspicious of Essay money both facility and speed. Good writing is English and writing essentially rewriting. I am positive of this.” — Roald Dahl. Part of my job entails training colleagues on Essay money valuation resume writing and general career counseling in order to be prepared to take on a competitive resume writing certification exam. Through this great experience, my team in the office has had a lot of time to develop and revise training for colleagues of various technical and writing skills. No matter what, the profile section of a resume has always been the How to to write a paper most time consuming. One newer strategy came a few weeks ago, that has worked fairly well for my own purposes for advice to coworkers and job seekers alike. [As a warning, all that follows is a journey down the Essay money dark caverns of my mind- or rather, my thought process as it relates to resume writing and strategies that have proven to be successful. Excuse my seemingly contradictory blog here, as I detail the benefits of being concise in writing assistance with, a long-winded post.] The profile statement is Essay money typically a paragraph section underneath a headline statement on a combination style resume.

Here is where the How to find to write first sentence has to be an all-encompassing marketing statement, highlighting the most significant selling points of the Essay money back valuation client that speaks to motivation research the employer’s needs. Every time we write one, I think of Essay back my time in a Shakespeare festival playing an in essay writing evil sister in a reenactment of King Lear. My close friend Stephanie played the money back other evil sister and we had to ‘fight’ over the same man. The first time running through the play quickly, it took an hour. Then for the audience, the goal was to do the Things to write and contrast play in a half hour. Then 10 minutes.

Then 5 minutes…. Then all the way down to 30 seconds. What started off as a snarky, witty back-and-forth between Stephanie and me became an all-out fight scene. We literally took each other down on stage, trying to get the essence of the evil sisters relationship conveyed under the time constraints. And I see you, staring at Essay valuation me right now, thinking, “Land the reading plane…” Well, consider the full 5-7 sentences of a full paragraph the 10 minute version. Money Back? The very first sentence is that 30 second version. The half hour may be the full resume and its accompanying documents, and the full hour would be the resume accompanied with the interview process until the motivation to write research hiring decision. Giving the Essay money back employer piece by piece what they need and no more is another exercise in time constraints. Giving them too much too soon could dance on the border of kalä±plarä± being irrelevant and the employer may lose interest or not see the worth amongst the wealth of information. To avoid resume jargon that doesn’t market a client well, I suggest the following.

When crafting the Summary- 1. Determine the unique selling points. 3-5 of them would be ideal. Selling points are what the client has to offer the Essay money employer. Did they make or save money? Did they manage a successful project? Implement a new program? Just complete a competitive training course? 2. List the unique selling points in order of significance. Determine which selling points have the most impact and lead with them.

Those of still impressive but lesser significance can help close the summary. With all else excluded, the first sentence has to function as a stand-alone sentence, able to market the client well as leading statement in case the employer only skims the resume. 3. Without using adjectives, articles, and to write essay on dogs, pronouns, write the unique selling points as full sentences. Describe the selling point in detail without any descriptive words. This may seem difficult at valuation first, but will be well worth it. Avoiding the use of adjectives makes writers use only the facts. By using only the Things to write and contrast facts, the true selling point is discerned without any “fluff” that could be considered debatable. Articles are all of our neuter words like “the,” “a/an,” etc. Removing pronouns helps lead with verbs, providing more impact to each sentence.

Take the example sentence of, “She had a great idea and developed a new marketing program that beat what most of the local competition was doing. Customers were prompted to come to the store because seasonal special discount coupons were mailed by Essay valuation her, and the customers gave her lots of positive feedback, they were so happy to Do my homework make have a surprise discount.” If we remove the adjectives, articles, and pronouns, it becomes, “Had idea and Essay money, developed new marketing program that beat what most of and writing past papers local competition was doing. Customers were prompted to come to back store because seasonal special discount coupons were mailed, and customers gave positive feedback, happy to have discounts.” 4. Use an includes your active voice. In the active voice, quite simply. the Essay back valuation subject does the action. English Reading And Writing Past Papers? Example – Jane developed a new marketing program. If we were to Essay valuation compared this with the passive voice, the target of the action becomes the subject. Example – A new marketing program was developed by Jane.

How do we place the above selling point in the active voice? We can break it down one line at motivation research a time. Essay Back? The first line, “Had idea and developed new marketing program that beat what most of local competition was doing,” is Things a compare and contrast essay already active because Jane/She is the understood subject. We dropped the Essay money pronoun in step 3. The next line, “Customers were prompted to come to store because seasonal special discount coupons were mailed, and customers came to store, gave positive feedback, happy to have surprise discounts,” is all passive. “Customers were prompted ” and “special discount coupons were mailed .” To change this to the active voice, the sentence can be changed to say “Mailed seasonal special discount coupons to customers, and customers came to store, gave positive feedback, happy to have surprise discounts.” 5. Be concise but speak the employer’s language.

Now that we have dropped some unnecessary words, have understood subjects in the active voice, and a list of selling points in essay on dogs, order of highest impact to lowest, we can edit the Essay money back valuation sentences further. On a resume, you want to use as few words as possible to say your point. To Write A Compare Essay On Dogs? Baroque, ornate sentences that flow on and on with continuous dependent clauses have no place on a resume. Let’s take a look at Essay money valuation how our summary line is going. Now we have, “Had idea and developed new marketing program that beat what most of to write research local competition was doing. Mailed seasonal special discount coupons to customers, and customers came to store, gave positive feedback, happy to have surprise discounts.” 35 words for one selling point is far too long. Having one selling point take more than one sentence is also not the best marketing idea if high-value sentences are to money be achieved.

Having a critical eye over includes what may be deemed necessary is crucial. Essay Money Valuation? The first sentence begins with, “Had idea and…” Had idea? Do we really need to say someone had an idea before they pursued something? It is pretty safe to assume that before someone develops something new, they originally had an idea to do it. Had an kalä±plarä± idea? Dropped.

“Developed new marketing program that beat what most of money local competition was doing.” This new marketing program is unique. Homework Make? No other local stores currently offer this promotion. The real “meat” of the sentence is that she “Developed (a) new marketing program.” What can we assume from the fact that it differs from what other stores currently offer? Although “unique” was used earlier, I would not recommend calling something “unique,” because everyone thinks they are unique. “Exclusive” perhaps? “Developed new and exclusive marketing program.” Now “new and” bothers me. It may even be safe to assume that when someone develops something, that it will be “new.” Typically, no one develops something that will not lead to some new change. “Developed exclusive marketing program.” There we go. Next sentence.

“Mailed seasonal special discount coupons to customers, and Essay, customers came to essay on dogs store, gave positive feedback, happy to have surprise discounts.” Comma, comma, comma. Valuation? Everything about Do my homework assignments this sentence is Essay dependent. “and customers came to store, gave positive feedback, happy to have surprise discounts,” is essentially the result of this new sales promotion. “Came to Critically thinking store” is just kind of, “meh” right? I did X and customers came. Since this is a sales-related statement, it may be more marketable to say, “increased traffic.” From increasing traffic, there may be room for more sales. Information on sales increases isn’t directly given to us, so we will continue with “increased traffic.” Similarly, “gave positive feedback, happy to have surprise discounts.” What we are really talking about here is “customer satisfaction.” Revisions in mind, we have now changed this too, “Developed exclusive marketing program.

Mailed seasonal special discount coupons to customers that increased traffic and customer satisfaction.” 6. Back? Make the sentences showcase abilities to offer an employer. The revision sounds very close to an achievement, which could be further developed into one for this resume. For the summary section, remove the specific details and save them for the achievements section. Revise the How to find motivation to write research sentence so it is something they can offer a prospective employer, and Essay money back valuation, the achievements section can become the “proof” section. “Proven ability to develop exclusive marketing programs. that increase traffic and customer satisfaction.”

Where is English reading it “ proven ” ? In the achievements section below. 7. Finishing touches: sprinkle in an adjective with a job title/phrase. As opposed to being adjective heavy and editing them out, we originally removed all or most adjectives in back valuation, step 3. Do My For Me Homework Assignments? It is much more difficult finding the “meat” of an ability/offering when it is flooded by Essay back meaningless adjectives, but one adjective at writing cover the end of fine tuning the sentence is Essay money valuation a great finishing touch. This adjective can be used to How to research describe the client, but avoid over-assuming or using cliches. “Self-motivated” or “Results-oriented” may be on a lot of money back “No” lists, but I think they are safe to use. It’s just a matter of quality, not quantity, when it comes to choosing these few adjectives.

For the job title/phrase, you can use the letter client’s current occupation, or a general term for their occupation- as long as it is not completely different from the Essay money job they are targeting. Overall, I would only avoid using the word “professional” as a standalone. If the person in our example is a manager in English, sales, we can call her a Sales Manager. Results-oriented Sales Manager with proven ability to develop exclusive marketing programs that increase traffic and customer satisfaction. 18 words, one sentence from 35 words and two sentences, with the major change being only an money valuation increase in impact. These steps take some time, but remember, like most writing- it is 20% drafting, and How to motivation to write a paper research, 80% revision.

Taking the time to critically revise makes a great impact on the resume’s marketability. If this sounds like a lot to handle, feel free to contact me so I can get you in touch with a CPRW from a local job center in valuation, your area.